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Volume 147, Issue 8

Association between intimin () and EspB gene subtypes in attaching and effacing strains isolated from diarrhoeic lambs and goat kids

The GenBank accession numbers for the sequences reported in this paper are AF253560 ( gene of strain CK379), AF253561 ( gene of strain CL559), and AF254454, AF254455, AF254456 and AF254457 ( nucleotide sequences of strains CL559, CL617, CK379 and CL398, respectively)

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Abstract

Attaching and effacing (AEEC) strains isolated from diarrhoeic lambs and goat kids were characterized for intimin () and EspB () gene subtypes by PCR and sequencing, and for genetic relatedness by PFGE. Fifty (23 ovine and 27 caprine) AEEC strains of 398 (246 ovine and 152 caprine) analysed were detected by colony blot hybridization. These strains were epidemiologically unrelated since they were isolated from different outbreaks of neonatal diarrhoea over a long period. Ovine AEEC strains belonged to serogroups O2, O4, O26, O80, O91 or were untypable, and caprine strains belonged to serogroups O3, O153 and O163. Two intimin subtypes were detected among the ovine and caprine strains studied. Most of the strains (43/50) had the β type intimin gene, but seven ovine strains possessed a variant γ type intimin gene (γ). Analysis of deduced amino acid sequences of the gene revealed that the sequences of β intimin of ovine and caprine strains were virtually identical to those of β intimin of rabbit EPEC, human EPEC clone 2 and swine AEEC, whereas the γ intimin present in seven ovine strains had 75–76% identity with γ intimin of human EHEC clone 1 strains, and 96% of identity with intimin of the human EHEC strain 95NR1 of serotype O111:H–. A PCR test was developed to identify the three different gene subtypes, of human EPEC clone 1 (α), of human EHEC clone 1 (γ) and of rabbit EPEC and human EPEC clone 2 (β). There was close correlation between the intimin β type and the β gene subtype in the ovine and caprine AEEC strains. The seven ovine strains possessing the γ intimin gene possessed the α gene subtype. None of the strains studied possessed the γ gene found in human O157:H7 EHEC strains. PFGE analysis of genomic DNA of selected strains showed a great diversity among strains. Cluster analysis of PFGE patterns showed greater divergence between strains with the γ intimin gene than between strains with the β intimin gene. This study showed that most of the AEEC strains isolated from diarrhoeic lambs and goat kids possessed β intimin and genes identical to those of rabbit EPEC, and they may be associated with enteric disease in small ruminants.

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Keyword(s): adherence , AE, attaching and effacing , AEEC, attaching and effacing E. coli , attaching and effacing Escherichia coli strains , CNF, cytotoxic necrotizing factor , diarrhoea , EHEC, enterohaemorrhagic E. coli , enteropathogenic E. coli , EPEC, enteropathogenic E. coli , Esp, EPEC secreted protein , HC, haemorrhagic colitis , HUS, haemolytic uraemic syndrome , LEE, locus for enterocyte effacement , pulsed field gel electrophoresis , Stx, Shiga toxin and VT, verotoxin
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2001-08-01
2024-04-25
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Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kidsThe GenBank accession numbers for the sequences reported in this paper are AF253560 (eae gene of strain CK379), AF253561 (eae gene of strain CL559), and AF254454, AF254455, AF254456 and AF254457 (espB nucleotide sequences of strains CL559, CL617, CK379 and CL398, respectively)
Microbiology 147, 2341 (2001); https://doi.org/10.1099/00221287-147-8-2341
/content/journal/micro/10.1099/00221287-147-8-2341
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