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Volume 147, Issue 8

Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46 Free

Abstract

Pilus-mediated adherence to mucosal epithelial cells is a critical step for to establish an infection in the human host. CD46, the defined receptor for the gonococcal pilus, is a complement-regulatory protein that is expressed on all human nucleated cells. It was observed that a piliated, Opa variant of gonococcal strain FA1090 adhered with different efficiencies to the human epithelial cell lines tested (Chang, ME180, HEC-1B and PC-3). Surprisingly, these differences in adherence levels did not correlate with levels of CD46 expressed by these cell lines. In fact, there was an inverse relationship between total surface-exposed CD46 and gonococcal adherence. Four major isoforms of CD46 are produced due to alternative RNA splicing of a surface-exposed region and the cytoplasmic tail. The relative isoform surface expression of each cell line was determined, and each was found to express different ratios of the four CD46 isoforms. No correlation could be derived between CD46 isoform surface expression and pilus-mediated gonococcal adherence, indicating that CD46 does not act as a classic receptor for gonococcal pili.

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  • Published Online:
Keyword(s): CFDA-SE, 5- and 6-carboxyfluorescein diacetate, succinimidyl ester , FACS, fluorescence-activated cell sorter , flow cytometry , fluorescent labelling , human epithelial cells and Neisseria gonorrhoeae
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2001-08-01
2024-04-16
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Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46
Microbiology 147, 2333 (2001); https://doi.org/10.1099/00221287-147-8-2333
/content/journal/micro/10.1099/00221287-147-8-2333
/content/journal/micro/10.1099/00221287-147-8-2333
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