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Volume 147, Issue 8

Characterization of the low-pH responses of using genomic DNA arrays Free

Abstract

is unique among bacterial pathogens in its ability to persist in the acidic environment of the human stomach. To identify genes responsive to low pH, the authors assembled a high-density array of PCR-amplified random genomic DNA. Hybridization of radiolabelled cDNA probes, prepared using total RNA from bacteria exposed to buffer at either pH 40 or pH 70, allowed both qualitative and quantitative information on differential gene expression to be obtained. A previously described low-pH-induced gene, , was identified together with several novel genes that may have relevance to the survival and persistence of in the gastric environment. These include genes encoding enzymes involved in LPS and phospholipid synthesis and , encoding a component of the protein export machinery. A hypothetical protein unique to (HP0681) was also found to be acid induced. Genes down-regulated at pH 40 include those encoding a sugar nucleotide biosynthesis protein, a flagellar protein and an outer-membrane protein. Differential gene expression was confirmed by total RNA slot-blot hybridization.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acid-induced gene expression , genomic DNA array and pathogenicity
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2001-08-01
2024-04-23
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Characterization of the low-pH responses of Helicobacter pylori using genomic DNA arrays
Microbiology 147, 2285 (2001); https://doi.org/10.1099/00221287-147-8-2285
/content/journal/micro/10.1099/00221287-147-8-2285
/content/journal/micro/10.1099/00221287-147-8-2285
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