1887

Abstract

Alternative sigma factors have been detected in the myxobacterium during indole-induced sporulation, fruiting body formation and heat shock using an antiserum raised against sigma factor SigB. The time course of gene expression was analysed by RT-PCR and by determining β-galactosidase activity during development in a merodiploid strain that harboured a fusion gene. Inactivation of the gene by insertion of the gene resulted in the loss of one sigma factor as shown by Western analysis. Neither fruiting body formation nor sporulation, nor the production of possible SigB targets, such as DnaK, GroEL or HspA, were affected.

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2001-08-01
2020-01-25
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References

  1. Apelian D., Inouye S.. 1990; Development-specific σ-factor essential for late-stage differentiation of Myxococcus xanthus. . Genes Dev4:1396–1403[CrossRef]
    [Google Scholar]
  2. Apelian D., Inouye S.. 1993; A new putative sigma factor of Myxococcus xanthus. . J Bacteriol175:3335–3342
    [Google Scholar]
  3. Bradford M. M.. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem72:248–254[CrossRef]
    [Google Scholar]
  4. Chomczynski P., Sacchi N.. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem162:156–159
    [Google Scholar]
  5. Gerth K., Metzger R., Reichenbach H.. 1993; Induction of myxospores in Stigmatella aurantiaca (myxobacteria) – inducers and inhibitors of myxospore formation, and mutants with a changed sporulation behaviour. J Gen Microbiol139:865–871[CrossRef]
    [Google Scholar]
  6. Harlow E., Lane D.. 1988; Antibodies. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Heidelbach M., Skladny H., Schairer H. U.. 1992; Purification of the DNA-dependent RNA polymerase from the myxobacterium Stigmatella aurantiaca. . J Bacteriol174:2733–2735
    [Google Scholar]
  8. Heidelbach M., Skladny H., Schairer H. U.. 1993; Heat shock and development induce synthesis of a low-molecular-weight stress-responsive protein in the myxobacterium Stigmatella aurantiaca. . J Bacteriol175:7479–7482
    [Google Scholar]
  9. Helmann J. D., Chamberlin M. J.. 1988; Structure and function of bacterial sigma factors. Annu Rev Biochem57:839–872[CrossRef]
    [Google Scholar]
  10. Ishihama A.. 1988; Promoter selectivity of prokaryotic RNA polymerases. Trends Genet4:282–286[CrossRef]
    [Google Scholar]
  11. Julien B., Kaiser A. D., Garza A.. 2000; Spatial control of cell differentiation in Myxococcus xanthus. . Proc Natl Acad Sci USA97:9098–9103[CrossRef]
    [Google Scholar]
  12. Kim S. K., Kaiser D., Kuspa A.. 1992; Control of cell density and pattern by intercellular signaling in Myxococcus development. Annu Rev Microbiol46:117–139[CrossRef]
    [Google Scholar]
  13. Kroos L., Kuspa A., Kaiser D.. 1986; A global analysis of developmentally regulated genes in Myxococcus xanthus. . Dev Biol 117:252–266[CrossRef]
    [Google Scholar]
  14. Kyhse-Andersen J.. 1984; Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods10:203–209[CrossRef]
    [Google Scholar]
  15. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685[CrossRef]
    [Google Scholar]
  16. Lonetto M., Gribskov M., Gross C. A.. 1992; The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol174:3843–3849
    [Google Scholar]
  17. de Lorenzo V., Herrero M., Jakubzik U., Timmis K. N.. 1990; Mini-Tn 5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram negative eubacteria. J Bacteriol172:6568–6572
    [Google Scholar]
  18. Losick R., Pero J.. 1981; Cascades of sigma factors. Cell25:582–584[CrossRef]
    [Google Scholar]
  19. Lünsdorf H., Schairer H. U., Heidelbach M.. 1995; Localization of the stress protein SP21 in indole-induced spores, fruiting bodies, and heat-shocked cells of Stigmatella aurantiaca. . J Bacteriol177:7092–7099
    [Google Scholar]
  20. McCarty J. S., Rudiger S., Schonfeld H. J., Schneider Mergener J., Nakahigashi K., Yura T., Bukau B.. 1996; Regulatory region C of the E. coli heat shock transcription factor, sigma32, constitutes a DnaK binding site and is conserved among eubacteria. J Mol Biol256:829–837[CrossRef]
    [Google Scholar]
  21. Miller J. H.. 1972; Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Neumann B., Pospiech A., Schairer H. U.. 1992; Rapid isolation of genomic DNA from Gram negative bacteria. Trends Genet8:332–333[CrossRef]
    [Google Scholar]
  23. Qualls G. T., Stephens K., White D.. 1978; Morphogenetic movements and multicellular development in the fruiting Myxobacterium, Stigmatella aurantiaca. . Dev Biol66:270–274[CrossRef]
    [Google Scholar]
  24. Reichenbach H., Dworkin M.. 1992; The myxobacteria. In The Procaryotes pp3416–3487 Edited by Balows A., Dworkin M., Harder W., Schleifer K.-H., Trüper H. G.. New York: Springer;
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74:5463–5467[CrossRef]
    [Google Scholar]
  27. Silakowski B., Ehret H., Schairer H. U.. 1998; fbfB , a gene encoding a putative galactose oxidase, is involved in Stigmatella aurantiaca fruiting body formation. J Bacteriol180:1241–1247
    [Google Scholar]
  28. Silakowski B., Pospiech A., Neumann B., Schairer H. U.. 1996; Stigmatella aurantiaca fruiting body formation is dependent on the fbfA gene encoding a polypeptide homologous to chitin synthases. J Bacteriol178:6706–6713
    [Google Scholar]
  29. Skladny H.. 1994; Sigmafaktoren des Myxobakteriums Stigmatella aurantiaca. PhD thesisUniversität Heidelberg. Heidelberg, Germany
    [Google Scholar]
  30. Skladny H., Heidelbach M., Schairer H. U.. 1992; Cloning and DNA sequence of sigB gene of Stigmatella aurantiaca.. Nucleic Acids Res20:6416[CrossRef]
    [Google Scholar]
  31. Skladny H., Heidelbach M., Schairer H. U.. 1994; Cloning and characterization of the gene encoding the major sigma factor of Stigmatella aurantiaca. . Gene143:123–127[CrossRef]
    [Google Scholar]
  32. Stamm I., Leclerque A., Plaga W.. 1999; Purification of cold-shock-like proteins from Stigmatella aurantiaca – molecular cloning and characterization of the cspA gene. Arch Microbiol172:175–181[CrossRef]
    [Google Scholar]
  33. Stragier P., Losick R.. 1990; Cascades of sigma factors revisited. Mol Microbiol4:1801–1806[CrossRef]
    [Google Scholar]
  34. Stragier P., Losick R.. 1996; Molecular genetics of sporulation in Bacillus subtilis. . Annu Rev Genet30:297–341[CrossRef]
    [Google Scholar]
  35. Ueki T., Inouye S.. 1997; Characterisation of sigma factors of Myxococcus xanthus . In Abstracts of the 24th Annual Meeting on the Biology of the Myxobacteria p1
    [Google Scholar]
  36. Ueki T., Inouye S.. 1998; A new sigma factor, SigD, essential for stationary phase is also required for multicellular differentiation in Myxococcus xanthus. . Genes Cells3:371–385[CrossRef]
    [Google Scholar]
  37. Voelz H., Reichenbach H.. 1969; Fine structure of fruiting bodies of Stigmatella aurantiaca (Myxobacterales). J Bacteriol99:856–866
    [Google Scholar]
  38. Wösten M. M. S. M.. 1998; Eubacterial sigma-factors. FEMS Microbiol Rev22:127–150[CrossRef]
    [Google Scholar]
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