1887

Abstract

Alternative sigma factors have been detected in the myxobacterium during indole-induced sporulation, fruiting body formation and heat shock using an antiserum raised against sigma factor SigB. The time course of gene expression was analysed by RT-PCR and by determining β-galactosidase activity during development in a merodiploid strain that harboured a fusion gene. Inactivation of the gene by insertion of the gene resulted in the loss of one sigma factor as shown by Western analysis. Neither fruiting body formation nor sporulation, nor the production of possible SigB targets, such as DnaK, GroEL or HspA, were affected.

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2001-08-01
2021-10-23
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