1887

Abstract

The effect of acetylene (CH) on aerobic and anaerobic ammonia oxidation by was investigated. Ammonia monooxygenase (AMO) was inhibited and a 27 kDa polypeptide (AmoA) was labelled during aerobic ammonia oxidation. In contrast, anaerobic, NO-dependent ammonia oxidation (NO/NO as oxidant) was not affected by acetylene. Further studies gave evidence that the inhibition as well as the labelling reaction were O-dependent. Cells pretreated with acetylene under oxic conditions were unable to oxidize ammonia with O as oxidant. After these cell suspensions were supplemented with gaseous NO, ammonia oxidation activity of about 140 μmol NH (g protein) h was detectable under both oxic and anoxic conditions. A significantly reduced acetylene inhibition of the ammonia oxidation activity was observed for cells incubated in the presence of NO. This suggests that NO and acetylene compete for the same binding site on AMO. On the basis of these results a new hypothetical model of ammonia oxidation by was developed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-8-2247
2001-08-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/8/1472247a.html?itemId=/content/journal/micro/10.1099/00221287-147-8-2247&mimeType=html&fmt=ahah

References

  1. Anderson K. K., Hooper A. B.. 1983; O2 and H2O are each the source of one O in \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(NO_{2}^{{-}}\) \end{document} produced from NH3 by Nitrosomonas ; 15N-NMR evidence. FEBS Lett164:236–240[CrossRef]
    [Google Scholar]
  2. Arciero D. M., Hooper A. B.. 1993; Hydroxylamine oxidoreductase from Nitrosomonas europaea is a multimer of an octa-heme subunit. J Biol Chem268:14645–14654
    [Google Scholar]
  3. Bergmann D. J., Arciero D. A., Hooper A. B.. 1994; Organization of the hao gene cluster of Nitrosomonas europaea : genes for two tetraheme c cytochromes. J Bacteriol176:3148–3153
    [Google Scholar]
  4. Bonner W. M., Laskey R. A.. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem46:83–88[CrossRef]
    [Google Scholar]
  5. Bradford M. M.. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem72:248–254[CrossRef]
    [Google Scholar]
  6. Dua R. D., Bhandari B., Nicholas D. J. D.. 1979; Stable isotope studies on the oxidation of ammonia to hydroxylamine by Nitrosomonas europaea. FEBS Lett106:401–404[CrossRef]
    [Google Scholar]
  7. Hyman M. R., Arp D. J.. 1990; The small-scale production of [U-14C]acetylene from Ba14CO3: Application to labeling of ammonia monooxygenase in autotrophic nitrifying bacteria. Anal Biochem190:348–353[CrossRef]
    [Google Scholar]
  8. Hyman M. R., Arp D. J.. 1992; 14C2H2- and 14CO2-labeling studies of the de novo synthesis of polypeptides by Nitrosomonas europaea during recovery from acetylene and light inactivation of ammonia monooxygenase. J Biol Chem267:1534–1545
    [Google Scholar]
  9. Hyman M. R., Arp D. J.. 1993; An electrophoretic study of the thermal-dependent and reductant-dependent aggregation of the 28 kDa component of ammonia monooxygenase from Nitrosomonas europaea. Electrophoresis 14:619–627[CrossRef]
    [Google Scholar]
  10. Hyman M. R., Arp D. J.. 1995; Effects of ammonia on the de novo synthesis of polypeptides in cells of Nitrosomonas europaea denied ammonia as an energy source. J Biol Chem177:4974–4979
    [Google Scholar]
  11. Hyman M. R., Wood P. M.. 1985; Suicidal inactivation and labelling of ammonia monooxygenase by acetylene. Biochem J227:719–725
    [Google Scholar]
  12. Hyman M. R., Page C. L., Arp D. J.. 1994; Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea. . Appl Environ Microbiol60:3033–3035
    [Google Scholar]
  13. Hynes R. K., Knowles R.. 1978; Inhibition by acetylene of ammonia oxidation in Nitrosomonas europaea. . FEMS Microbiol Lett4:319–321[CrossRef]
    [Google Scholar]
  14. Pires M., Rossi M. J., Ross D. S.. 1994; Kinetic and mechanistic aspects of the NO oxidation by O2 in aqueous phase. Int J Chem Kinet26:1207–1227[CrossRef]
    [Google Scholar]
  15. Rees M., Nason A.. 1966; Incorporation of atmospheric oxygen into nitrite formed during ammonia oxidation by Nitrosomonas europaea. . Biochim Biophys Acta 113:398–401[CrossRef]
    [Google Scholar]
  16. Sayavedra-Soto L. A., Hommes N. G., Arp D. J.. 1994; Characterization of the gene encoding hydroxylamine oxidoreductase in Nitrosomonas europaea. . J Bacteriol176:504–510
    [Google Scholar]
  17. Schmidt I., Bock E.. 1997; Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha. . Arch Microbiol167:106–111[CrossRef]
    [Google Scholar]
  18. Schmidt I., Bock E.. 1998; Anaerobic ammonia oxidation by cell-free extracts of Nitrosomonas eutropha. . Antonie Leeuwenhoek 73:271–278[CrossRef]
    [Google Scholar]
  19. Suzuki I., Kwok S.-C.. 1970; Cell-free ammonia oxidation by Nitrosomonas europaea extracts: Effects of polyamines, Mg2+ and albumin. Biochem Biophys Res Commun39:950–955[CrossRef]
    [Google Scholar]
  20. Suzuki I., Kwok S.-C., Dular U., Tsang D. C. Y.. 1981; Cell-free ammonia-oxidizing system of Nitrosomonas europaea : general conditions and properties. Can J Biochem59:477–483[CrossRef]
    [Google Scholar]
  21. Wood P. M.. 1986; Nitrification as a bacterial energy source. In Nitrification pp39–62 Edited by Prosser J. I.. Washington, DC: IRL Press;
    [Google Scholar]
  22. Zart D., Bock E.. 1998; High rate of aerobic nitrification and denitrification by Nitrosomonas eutropha grown in a fermentor with complete biomass retention in the presence of gaseous NO2 or NO. Arch Microbiol169:282–286[CrossRef]
    [Google Scholar]
  23. Zart D., Schmidt I., Bock E.. 2000; Significance of gaseous NO for ammonia oxidation by Nitrosomonas eutropha. . Antonie Leeuwenhoek77:49–55[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-8-2247
Loading
/content/journal/micro/10.1099/00221287-147-8-2247
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error