1887

Abstract

’ HBP1 degrades 2-hydroxybiphenyl (2-HBP) and 2,2′-diHBP by employing a -cleavage pathway encoded by the genes. The regulatory gene , located directly upstream of the genes and oriented in the opposite direction, encodes a transcription activator protein belonging to the so-called XylR/DmpR subclass within the NtrC family. HbpR activates transcription from two separate σ-dependent promoters upstream of the and the genes, in the presence of the pathway substrates 2-HBP and 2,2′-diHBP. The DNA region upstream of the gene displays an unusual organization, containing two adjacent 03 kb regions that share 71% sequence identity. The DNA region most proximal to the promoter harbours one pair of putative upstream activating sequences (UASs C-1/C-2) and a small cryptic ORF that shows homology to itself. The second, more distal, region contains a second pair of putative UASs (UASs C-3/4) and the 5′-part of the gene. Transcriptional fusions in between different deletions of the intergenic region and the genes for bacterial luciferase revealed that most if not all of the transcriptional output from the promoter is mediated from the proximal UASs C-1/C-2. However, when the UASs C-1/C-2 were deleted and UASs C-3/C-4 were placed in an appropriate position with respect to the promoter region, the promoter was still inducible with 2-HBP, albeit at a lower level. Transcription studies in and ‘’ revealed that the divergently oriented gene is expressed constitutively from a σ-dependent promoter situated within the cryptic ORF. The presence of UAS pair C-3/C-4 mediated a slightly higher promoter activity for transcription of .

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2001-08-01
2020-01-25
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