1887

Abstract

Although gene clusters for the degradation of biphenyls and polychlorobiphenyls have been extensively characterized, comparatively little is known about the regulation of their expression. In the present work, different aspects of transcription of the locus of the potent polychlorobiphenyl degrader sp. strain LB400 were investigated. An RNA blot analysis of the entire gene cluster revealed that the transcription of all genes encoding biphenyl catabolic enzymes responded similarly to the presence of biphenyl, succinate or a mixture of the two. One region of the locus, encompassing ORF0, was separately transcribed and differently regulated. A single start position was mapped for this monocistronic transcript. Synthesis of the adjacent RNA, encoding subunits of biphenyl dioxygenase, was strongly biphenyl-inducible. In this case, four major 5′-ends were mapped between 25 and 70 bp upstream of the start codon of gene . Sequence elements between approximately positions 710 and 1080 upstream were required for full functioning of the respective promoter(s) (P). ORF0 mutants of strain LB400 retained the ability to grow on biphenyl, but showed decreased concentrations of RNA and decreased expression in strains harbouring a reporter system with a transcriptional fusion. This effect was compensated by the introduction of an intact ORF0 , indicating that the ORF0 gene product mediates activation of P.

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2001-08-01
2020-01-20
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