1887

Abstract

Expression of the phenol degradation pathway in strain PaW85 requires coordinated transcription of the plasmid-borne operon encoding catechol 1,2-dioxygenase and phenol monooxygenase, respectively, and the chromosomally encoded catechol degradation operon. Transcriptional activation from the and promoters is regulated by CatR and the catechol degradation pathway intermediate ,-muconate. Here it is shown that physiological control mechanisms are superimposed on this regulatory system. Transcriptional activation from the and promoters is growth-phase-regulated in cells grown on rich medium (LB medium). CatR-mediated transcription from these promoters is silenced on rich medium until the transition from exponential to stationary phase. A slight positive effect (threefold) of stationary-phase-specific sigma factor σ on transcription from the promoter was observed. Expression of the promoter was not influenced by the activity of this sigma factor. In contrast to rich growth medium, transcription from the and promoters in minimal medium containing a mixture of glucose and sodium benzoate was rapidly induced in exponential culture. It was shown that the presence of amino acids in the culture medium causes exponential silencing of the and promoters. The possibility that a hypothetical repressor protein could be involved in physiological control of transcription from the and promoters is discussed.

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2001-08-01
2019-10-22
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