RT Journal Article SR Electronic(1) A1 Clément, Pascale A1 Pieper, Dietmar H A1 González, BernardoYR 2001 T1 Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acidThe GenBank accession numbers for the 3115 nt BamHI-F and 2833 nt EcoRI-F fragments of pJP4 and the 4037 nt EcoRI-E′ fragment of pJP4-F3 are AF225972, AF225973 and AF225974, respectively. JF Microbiology, VO 147 IS 8 SP 2141 OP 2148 DO https://doi.org/10.1099/00221287-147-8-2141 PB Microbiology Society, SN 1465-2080, AB Ralstonia eutropha JMP134(pJP4) is able to grow on minimal media containing the pollutants 3-chlorobenzoate (3-CB) or 2,4-dichlorophenoxyacetate (2,4-D). tfd genes from the 88 kb plasmid pJP4 encode enzymes involved in the degradation of these compounds. During growth of strain JMP134 in liquid medium containing 3-CB, a derivative strain harbouring a ∼∼95 kb plasmid was isolated. This derivative, designated JMP134(pJP4-F3), had an improved ability to grow on 3-CB, but had lost the ability to grow on 2,4-D. Sequence analysis of pJP4-F3 indicated that the plasmid had undergone a deletion of ∼∼16 kb, which included the tfdA–tfdS intergenic region, spanning the tfdA gene to a previously unreported IS1071 element. The loss of the tfdA gene explains the failure of the derivative to grow on 2,4-D. A ∼∼23 kb duplication of the region spanning tfdR-tfdD II C II E II F II-tfdB II-tfdK-ISJP4-tfdT-tfdC I D I E I F I-tfdB I, giving rise to a 51-kb-long inverted repeat, was also observed. The increase in gene copy number for the tfdCD(DC)EF gene cluster may provide an explanation for the derivative strain’s improved growth on 3-CB. These observations are additional examples of the metabolic plasticity of R. eutropha JMP134, one of the more versatile pollutant-degrading bacteria., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-8-2141