1887

Abstract

Simple sequence repeats, due to their high variability, are widely used for molecular epidemiology of pathogenic micro-organisms. However, their usefulness is restricted by their high instability and low information content. Here, a locus, CKTNR, in the fungal pathogen is described which displays considerable sequence, as well as length, heterogeneity. Alleles of this locus, which contains a degenerate trinucleotide repeat, appear to be stable. The CKTNR polymorphism could serve as the basis for a molecular typing system of . Furthermore, analysis of the CKTNR allele distribution suggested that reproduces mainly clonally.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-8-2021
2001-08-01
2020-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/8/1472021a.html?itemId=/content/journal/micro/10.1099/00221287-147-8-2021&mimeType=html&fmt=ahah

References

  1. Abbas J., Bodey G. P., Hanna H. A., Mardani M., Girgawy E., Abi-Said D., Whimbey E., Hachem R., Raad I.. 2000; Candida krusei fungemia. An escalating serious infection in immunocompromised patients. Arch Intern Med160:2659–2664[CrossRef]
    [Google Scholar]
  2. Barnett J. A., Payne R. W., Yarrow D.. 2000; Yeasts: Characteristics and Identification , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Becker K., Badehorn D., Deiwick S., Peters G., Fegeler W.. 2000; Molecular genotyping of Candida species with special respect to Candida (Torulopsis) glabrata strains by arbitrarily primed PCR. J Med Microbiol49:575–581
    [Google Scholar]
  4. van Belkum A.. 1999; The role of short sequence repeats in epidemiologic typing. Curr Opin Microbiol2:306–311[CrossRef]
    [Google Scholar]
  5. Caetano-Anolles G.. 1993; Amplifying DNA with arbitrary oligonucleotide primers. PCR Methods Appl3:85–94[CrossRef]
    [Google Scholar]
  6. Carlotti A., Chaib F., Couble A., Bourgeois N., Blanchard V., Villard J.. 1997; Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1. J Clin Microbiol35:1337–1343
    [Google Scholar]
  7. Field D., Wills C.. 1998; Abundant microsatellite polymorphism in Saccharomyces cerevisiae , and the different distributions of microsatellites in eight prokaryotes and S. cerevisiae , result from strong mutation pressures and a variety of selective forces. Proc Natl Acad Sci USA95:1647–1652[CrossRef]
    [Google Scholar]
  8. Field D., Eggert L., Metzgar D., Rose R., Wills C.. 1996; Use of polymorphic short and clustered coding-region microsatellites to distinguish strains of Candida albicans. FEMS Immunol Med Microbiol 15:73–79[CrossRef]
    [Google Scholar]
  9. Guo S. W., Thompson E. A.. 1992; Performing the exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics48:361–372[CrossRef]
    [Google Scholar]
  10. Hunter P. R.. 1991; A critical review of typing methods for Candida albicans and their applications. Crit Rev Microbiol17:417–434[CrossRef]
    [Google Scholar]
  11. Hunter P. R., Gaston M. A.. 1988; Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol26:2465–2466
    [Google Scholar]
  12. Kurtzman C. P., Smiley M. J.. 1976; Heterothallism in Pichia kudriavzevii and Pichia terricola. Antonie Leeuwenhoek. 42355–363[CrossRef]
  13. Kurtzman C. P., Smiley M. J., Johnson C. J.. 1980; Emendation of the genus Issatchenkia Kudriavzev and comparison of species by deoxyribonucleic acid reassociation, mating reaction, and ascospore ultrastructure. Int J Syst Bacteriol30:503–513[CrossRef]
    [Google Scholar]
  14. Lunel F. V., Licciardello L., Stefani S., Verbrugh H. A., Melchers W. J., Meis J. F., Scherer S., van Belkum A.. 1998; Lack of consistent short sequence repeat polymorphisms in genetically homologous colonizing and invasive Candida albicans strains. J Bacteriol180:3771–3778
    [Google Scholar]
  15. Metzgar D., Field D., Haubrich R., Wills C., van Belkum A.. 1998; Random amplification of polymorphic DNA and microsatellite genotyping of pre- and posttreatment isolates of Candida spp. from human immunodeficiency virus-infected patients on different fluconazole regimens. J Clin Microbiol36:2308–2313
    [Google Scholar]
  16. Metzgar D., Thomas E., Davis C., Field D., Wills C.. 2001; The microsatellites of Escherichia coli : rapidly evolving repetitive DNAs in a non-pathogenic prokaryote. Mol Microbiol39:183–190[CrossRef]
    [Google Scholar]
  17. Meunier J. R., Grimont P. A.. 1993; Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res Microbiol144:373–379[CrossRef]
    [Google Scholar]
  18. Orti G., Pearse D. E., Avise J. C.. 1997; Phylogenetic assessment of length variation at a microsatellite locus. Proc Natl Acad Sci USA94:10745–10749[CrossRef]
    [Google Scholar]
  19. Raymond M., Rousset F.. 1995; genepop: population genetics software for exact tests and ecumencicsm. J Heredity86:248–249
    [Google Scholar]
  20. Rex J. H., Walsh T. J., Sobel J. D., Filler S. G., Pappas P. G., Dismukes W. E., Edwards J. E.. 2000; Practice guidelines for the treatment of candidiasis. Infectious Diseases Society of America. Clin Infect Dis30:662–678[CrossRef]
    [Google Scholar]
  21. Rolfsmeier M. L., Lahue R. S.. 2000; Stabilizing effects of interruptions on trinucleotide repeat expansions in Saccharomyces cerevisiae. Mol Cell Biol 20:173–180[CrossRef]
    [Google Scholar]
  22. Samaranayake Y. H., Samaranayake L. P.. 1994; Candida krusei : biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen. J Med Microbiol41:295–310[CrossRef]
    [Google Scholar]
  23. Soll D. R.. 2000; The ins and outs of DNA fingerprinting the infectious fungi. Clin Microbiol Rev13:332–370[CrossRef]
    [Google Scholar]
  24. Strand M., Prolla T. A., Liskay R. M., Petes T. D.. 1993; Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. Nature365:274–276[CrossRef]
    [Google Scholar]
  25. Taylor J. W., Geiser D. M., Burt A., Koufopanou V.. 1999; The evolutionary biology and population genetics underlying fungal strain typing. Clin Microbiol Rev12:126–146
    [Google Scholar]
  26. Tibayrenc M., Kjellberg F., Arnaud J., Oury B., Breniere S. F., Darde M. L., Ayala F. J.. 1991; Are eukaryotic microorganisms clonal or sexual? A population genetics vantage. Proc Natl Acad Sci USA88:5129–5133[CrossRef]
    [Google Scholar]
  27. Tyler K. D., Wang G., Tyler S. D., Johnson W. M.. 1997; Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens. J Clin Microbiol35:339–346
    [Google Scholar]
  28. Welsh J., McClelland M.. 1990; Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res18:7213–7218[CrossRef]
    [Google Scholar]
  29. Whelan W. L., Kwon-Chung K. J.. 1988; Auxotrophic heterozygosities and the ploidy of Candida parapsilosis and Candida krusei. J Med Vet Mycol 26:163–171[CrossRef]
    [Google Scholar]
  30. Young E. T., Sloan J. S., Van Riper K.. 2000; Trinucleotide repeats are clustered in regulatory genes in Saccharomyces cerevisiae. Genetics1541053–1068
    [Google Scholar]
  31. Zeng S., Wu L. C., Lehmann P. F.. 1996; Random amplified polymorphic DNA analysis of culture collection strains of Candida species. J Med Vet Mycol34:293–297[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-8-2021
Loading
/content/journal/micro/10.1099/00221287-147-8-2021
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error