1887

Abstract

Shiga-toxin-2 ( )-encoding bacteriophages were isolated from Norwegian O157:H7 isolates of cattle and human origin. The phages were characterized by restriction enzyme analysis, hybridization with probes for toxin genes and selected phage DNA such as the gene, integrase gene and IS, and by PCR studies and partial sequencing of selected DNA regions in the integrase to region of the phages. The -phage-containing bacteria from which inducible phages were detected produced similar amounts of toxin, as shown by a Vero cell assay. The results indicate that the population of -carrying phages is heterogeneous, although the phages from epidemiologically linked O157:H7 isolates were similar. There appears to have been frequent recombination of phages with other lambdoid phages.

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2001-07-01
2019-09-20
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