1887

Abstract

For plasmids derived from bacteriophage λ, the initiation of bidirectional DNA replication from λ depends on the stimulation of transcription from the promoter by the host replication initiator protein DnaA. Certain (ts) mutants cannot be transformed by wild-type λ plasmids even at the temperature permissive to cell growth. This plasmid–host incompatibility appeared to be due to inefficient stimulation of transcription from the promoter by the mutant DnaA protein. This paper shows that there is a second mechanism for the incompatibility between λ plasmids and (ts) hosts, exemplified in this study by the mutant. This is based on the competition between the λ P protein and the host DnaA and DnaC proteins for DnaB helicase. Both mechanisms must be operative for the incompatibility.

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2001-07-01
2020-01-29
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