1887

Abstract

For plasmids derived from bacteriophage λ, the initiation of bidirectional DNA replication from λ depends on the stimulation of transcription from the promoter by the host replication initiator protein DnaA. Certain (ts) mutants cannot be transformed by wild-type λ plasmids even at the temperature permissive to cell growth. This plasmid–host incompatibility appeared to be due to inefficient stimulation of transcription from the promoter by the mutant DnaA protein. This paper shows that there is a second mechanism for the incompatibility between λ plasmids and (ts) hosts, exemplified in this study by the mutant. This is based on the competition between the λ P protein and the host DnaA and DnaC proteins for DnaB helicase. Both mechanisms must be operative for the incompatibility.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-7-1923
2001-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/7/1471923a.html?itemId=/content/journal/micro/10.1099/00221287-147-7-1923&mimeType=html&fmt=ahah

References

  1. Allen G. C. Jr, Kornberg A. 1991; Fine balance in the regulation of DnaB helicase by DnaC protein in replication in Escherichia coli. J Biol Chem 266:22096–22101
    [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characteriazation of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113 [CrossRef]
    [Google Scholar]
  3. Boyd A. C., Sherratt D. J. 1995; The pCLIP plasmids: versatile cloning vectors based on the bacteriophage lambda origin of replication. Gene 153:57–62 [CrossRef]
    [Google Scholar]
  4. von Freiesleben U., Rasmussen K. V., Schaechter M. 1994; SeqA limits DnaA activity in replication from oriC in Escherichia coli. Mol Microbiol 14:763–772 [CrossRef]
    [Google Scholar]
  5. Hansen F. G., Koefoed S., Atlung T. 1992; Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli. Mol Gen Genet 234:14–21
    [Google Scholar]
  6. Herman-Antosiewicz A., Taylor K, Węgrzyn A., Węgrzyn G. 1998a; DnaA-mediated regulation of phage λ-derived replicons in the absence of p R and Cro function. Virology 249:98–107 [CrossRef]
    [Google Scholar]
  7. Herman-Antosiewicz A., Taylor K, Śrutkowska S., Węgrzyn G. 1998b; Replication and maintenance of λ plasmids devoid of the Cro repressor autoregulatory loop in Escherichia coli. Plasmid 40113–125 [CrossRef]
    [Google Scholar]
  8. Jensen K. F. 1993; The Escherichia coli ‘‘wild types’’ W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J Bacteriol 175:3401–3407
    [Google Scholar]
  9. Konieczny I., Marszałek J. 1995; The requirement for molecular chaperones in λ DNA replication is reduced by mutation π in λ P gene which weakens the interaction between λP protein and DnaB helicase. J Biol Chem 270:9792–9799 [CrossRef]
    [Google Scholar]
  10. Konopa G., Szalewska-Pałasz A., Schmidt A., Messer W, Śrutkowska S., Węgrzyn G. 1999; The presence of two DnaA-binding sequences is required for efficient interaction of the Escherichia coli DnaA protein with each particular weak DnaA box region. FEMS Microbiol Lett 174:25–31 [CrossRef]
    [Google Scholar]
  11. Kornberg A., Baker T. A. 1992 DNA Replication New York: W. H. Freeman;
    [Google Scholar]
  12. Kur J., Taylor K, Górska I. 1987; Escherichia coli dnaA initiation function is required for replication of plasmids derived from coliphage lambda. J Mol Biol 198:203–210 [CrossRef]
    [Google Scholar]
  13. Lu M., Campbell J. L., Boye E., Kleckner N. 1994; SeqA: a negative modulator of replication initiation in E. coli. Cell 77:413–426 [CrossRef]
    [Google Scholar]
  14. Marszałek J., Kaguni J. M. 1994; DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. J Biol Chem 269:4883–4890
    [Google Scholar]
  15. Messer W., Blaesing F., Jakimowicz D. 9 other authors 2001; Bacterial replication initiator DnaA. Rules for DnaA binding and roles of DnaA in origin unwinding and helicase loading. Biochimie 83:5–13 [CrossRef]
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Seitz H., Weigel C., Messer W. 2000; The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli. Mol Microbiol 37: , 1270–1279 [CrossRef]
    [Google Scholar]
  18. Sutton M. D., Carr K. M., Vicente M., Kaguni J. M. 1998; Escherichia coli DnaA protein. The N-terminal domain and loading of DnaB helicase at the E. coli chromosomal origin. J Biol Chem 273:34255–34262 [CrossRef]
    [Google Scholar]
  19. Szalewska-Pałasz A., Węgrzyn G. 1994; An additional role of transcriptional activation of ori λ in the regulation of λ plasmid replication in Escherichia coli. Biochem Biophys Res Commun 205:802–806 [CrossRef]
    [Google Scholar]
  20. Szalewska-Pałasz A., Węgrzyn A., Herman A., Węgrzyn G. 1994; The mechanism of the stringent control of λ plasmid DNA replication. EMBO J 13:5779–5785
    [Google Scholar]
  21. Szalewska-Pałasz A., Taylor K, Węgrzyn A., Błaszczak A., Węgrzyn G. 1998a; DnaA-stimulated transcriptional activation of ori λ: Escherichia coli RNA polymerase β subunit as a transcriptional activator contact site. Proc Natl Acad Sci USA 95:4241–4246 [CrossRef]
    [Google Scholar]
  22. Szalewska-Pałasz A., Lemieszek E., Pankiewicz A., Helinski D. R, Węgrzyn A., Węgrzyn G. 1998b; Escherichia coli dnaA gene function and bacteriophage λ replication. FEMS Microbiol Lett 167:27–32
    [Google Scholar]
  23. Szalewska-Pałasz A., Weigel C., Speck C., Konopa G., Lurz R., Marszałek J., Taylor K., Messer W, Śrutkowska S., Węgrzyn G. 1998c; Interaction of the Escherichia coli DnaA protein with bacteriophage λ DNA. Mol Gen Genet 259:679–688 [CrossRef]
    [Google Scholar]
  24. Taylor K., Węgrzyn G. 1995; Replication of coliphage lambda DNA. FEMS Microbiol Rev 17:109–119 [CrossRef]
    [Google Scholar]
  25. Taylor K., Węgrzyn G. 1998; Regulation of bacteriophage λ replication. In Molecular Microbiology pp 81–97 Edited by Busby S. J. W., Thomas C. M., Brown N. L. Berlin-Heidelberg: Springer;
    [Google Scholar]
  26. Torheim N. K., Boye E., Lobner-Olsen A., Stokke T., Skarstad K. 2000; The Escherichia coli SeqA protein destabilizes mutant DnaA204 protein. Mol Microbiol 37:629–638
    [Google Scholar]
  27. Węgrzyn G. Taylor K. 1992; Inheritance of the replication complex by one of two daughter copies during λ plasmid replication in Escherichia coli. J Mol Biol 226:681–688 [CrossRef]
    [Google Scholar]
  28. Węgrzyn A., Węgrzyn G. 2001; Inheritance of the replication complex: a unique or common phenomenon in the control of DNA replication?. Arch Microbiol 175:86–93 [CrossRef]
    [Google Scholar]
  29. Węgrzyn A., Węgrzyn G., Taylor K. 1995; Plasmid and host functions required for λ plasmid replication carried out by the inherited replication complex. Mol Gen Genet 247:501–508 [CrossRef]
    [Google Scholar]
  30. Węgrzyn A., Węgrzyn G. Herman A., Taylor K. 1996a; Protein inheritance: λ plasmid replication perpetuated by the heritable replication complex. Genes Cells 1:953–963 [CrossRef]
    [Google Scholar]
  31. Węgrzyn G., Węgrzyn A., Pankiewicz A. Taylor K. 1996b; Allele specificity of the Escherichia coli dnaA gene function in the replication of plasmids derived from phage λ. Mol Gen Genet 252:580–586
    [Google Scholar]
  32. Węgrzyn A., Wróbel B., Węgrzyn G. 1999; Altered biological properties of cell membranes in Escherichia coli dnaA and seqA mutants. Mol Gen Genet 261:762–769 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-7-1923
Loading
/content/journal/micro/10.1099/00221287-147-7-1923
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error