1887

Abstract

A physical and genetic map of the NCP 262 chromosome was constructed. The order of macrorestriction fragments was determined by analysing fragments generated after single and double digestion with the restriction enzymes HII, I-I, 8387I, II and I and separation by PFGE. The I-I backbone of was constructed by indirect end-labelling with - and 3′ -specific probes located on either side of the I-I site in the operon, and reciprocal separation of HII and I-I digestion products by two-dimensional PFGE. The positions of HII fragments on the physical map were determined using a library of linking clones containing HII cleavage sites. The size of the circular genome was estimated to be 53 Mb with a mean resolution of approximately 140 kb. The chromosome of contains 12 operons, located on 46% of the chromosome, which are transcribed divergently from the deduced origin of replication. The genetic map was constructed by determining the location of 28 genes involved in house-keeping, heat-shock response, sporulation, electron transfer and acid- and solvent-formation. Comparison of the genetic map with those of the spore-forming bacteria , , and indicated to be most similar to the latter two species, with the order of the genes within the and loci being conserved.

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2001-07-01
2020-01-19
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