1887

Abstract

The immune inhibitor A (InhA) metalloprotease from specifically cleaves antibacterial proteins produced by the insect host, suggesting that it may contribute to the overall virulence of . The transcriptional regulation of the gene in both and was investigated. Using a transcriptional fusion, it was shown that expression is activated at the onset of sporulation. However, the transcriptional start site of is similar to σ-dependent promoters, and deletion of the sporulation-specific sigma factors σ or σ had no effect on expression in . The DNA region upstream from contains two genes encoding polypeptides similar to the SinI and SinR regulators of . SinR is a DNA-binding protein regulating gene expression and SinI inhibits SinR activity. Overexpression of the genes affects the expression of the transcriptional fusion in : early induction of expression was observed when was overexpressed, whereas expression was reduced in a strain overexpressing , suggesting that transcription is repressed, directly or indirectly, by SinR. transcription was greatly reduced in and mutants. Analysis of the expression in and mutants of indicates that the Spo0A-dependent regulation of expression depends on AbrB, which is known to regulate expression of transition state and sporulation genes in .

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2001-07-01
2019-09-16
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