1887

Abstract

Ohr (a protein involved in organic peroxide protection) and OsmC (an osmotically inducible protein of unknown function) are related proteins. Database searches and phylogenetic analyses reveal that Ohr and OsmC homologues cluster into two related subfamilies of proteins widely distributed in both Gram-negative and Gram-positive bacteria. To determine if these two subfamilies are functionally distinct, and in (a bacterium with one representative from each subfamily) were analysed. Only mutants are hypersensitive to organic peroxide, and this phenotype can be restored by complementation with but not . In addition, expression of was highly induced only by organic peroxides, and not by other oxidants or stresses. In contrast, was induced by ethanol and osmotic stress. A similar pattern of regulation was observed for Ohr and OsmC homologues in the Gram-positive bacterium , though uninduced expression was much higher and induction lower in this species. These data clearly support the conclusion that Ohr and OsmC define two functionally distinct subfamilies with distinct patterns of regulation.

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2001-07-01
2019-12-14
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