1887

Abstract

LysE of belongs to a large new superfamily of translocators whose members are probably all involved in the export of small solutes. Here, the transcript initiation site of , and its divergently transcribed regulator gene, , are identified. Single-copy transcriptional fusions of with , and titration experiments, show that LysG is the positive regulator of expression enabling its up to 20-fold induction. This induction requires the presence of a coinducer, which is either intracellular L-lysine, or L-arginine. A competition experiment showed that LysE exports these two basic amino acids at comparable rates of about 075 nmol min (mg dry wt). Although L-histidine and L-citrulline also act as coinducers of expression, these two amino acids are not exported by LysE. As is evident from the analysis of a deletion mutant, the physiological role of the system is to prevent bacteriostasis due to elevated L-lysine or L-arginine concentrations that arise during growth in the presence of peptides or in mutants possessing a deregulated biosynthesis pathway. has additional export activities other than those of LysE for exporting L-histidine, L-citrulline and L-ornithine.

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2001-07-01
2020-09-22
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