1887

Abstract

Four laccase isozyme genes, Psc , , and have been cloned from the edible mushroom, . The genes display a high degree of homology with other basidiomycete laccases (55–99%) at the amino acid level. Of the laccase genes isolated, Psc and displayed the highest degree of similarity (85% at the amino acid level), while Psc showed the highest degree of divergence, exhibiting only 52–57% amino acid similarity to the other laccase gene sequences. Laccase activity in is affected by nutrient nitrogen and carbon, and by the addition of copper and manganese to the growth medium. In addition, 2,5-xylidine, ferulic acid, veratric acid and 1-hydroxybenzotriazole induced laccase activity in the fungus. Induction of individual laccase isozyme genes by carbon, nitrogen, copper, manganese and the two aromatic compounds, 2,5-xylidine and ferulic acid, occured at the level of gene transcription. While Psc transcript levels appeared to be constitutively expressed, transcript levels for the other laccase isozyme genes, , and , were differentially regulated under the conditions tested.

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2001-07-01
2019-12-13
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