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Volume 147, Issue 7

Closely related genotypes show remarkably different depth distributions in two oceanic regions as revealed by hybridization using 16S rRNA-targeted oligonucleotides

The GenBank accession numbers for the sequences reported in this paper are AF311217 (RCC278, EQPAC1), AF311218 (RCC277, NATL1MIT), AF311219 (RCC280, NATL2B), AF311220 (RCC264, TAK9803-2), AF311291 (WH7803), AF311292 (WH8018) and AF311293 (WH8103).

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Abstract

An hybridization method was applied to the identification of marine cyanobacteria assignable to the genus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed to give positive signals. Genotype-specific oligonucleotides for high light (HL)- and low light (LL)-adapted members of this genus were identified by 16S rRNA sequence analyses and their specificities confirmed in whole-cell hybridizations with cultured strains of Chisholm , 1992 , sp. and sp. hybridization of these genotype-specific probes to field samples from stratified water bodies collected in the North Atlantic Ocean and the Red Sea allowed a rapid assessment of the abundance and spatial distribution of HL- and LL-adapted . In both oceanic regions the LL-adapted populations were localized in deeper water whereas the HL-adapted populations were not only distinct in each region but also exhibited strikingly different depth distributions, HLI being confined to shallow water in the North Atlantic, in contrast to HLII, which was present throughout the water column in the Red Sea.

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Keyword(s): DAPI, 4’,6-diamidino-2-phenylindole , 16S rRNA , cyanobacteria , HL, high light , HRP, horseradish peroxidase , LL, low light , oligonucleotide probes , Red Sea , TETON, 4-(1,4,7,10-tetra-oxadecyl)-1-naphthol , TSA and TSA, tyramide signal amplification
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2001-07-01
2024-04-19
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Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotidesThe GenBank accession numbers for the sequences reported in this paper are AF311217 (RCC278, EQPAC1), AF311218 (RCC277, NATL1MIT), AF311219 (RCC280, NATL2B), AF311220 (RCC264, TAK9803-2), AF311291 (WH7803), AF311292 (WH8018) and AF311293 (WH8103).
Microbiology 147, 1731 (2001); https://doi.org/10.1099/00221287-147-7-1731
/content/journal/micro/10.1099/00221287-147-7-1731
/content/journal/micro/10.1099/00221287-147-7-1731
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