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Volume 147, Issue 6

Population genetics of in the southern part of Switzerland analysed by sequencing of four housekeeping genes (, , and ), and by , , and IS genotyping

The GenBank accession numbers for the sequences reported in this paper are AY004351–AY004662

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Abstract

The population biology of 78 strains (71 from Swiss Italian, 4 from East Asian and 3 from South African patients) was investigated by sequence analysis of four housekeeping genes: , , and . The genotype, the presence of and IS, the allelic type, and the resistance to metronidazole, clarithromycin and amoxycillin were determined. A high percentage of DNA polymorphic sites (198% for , 213% for , 237% for and 203% for ) was found. The phylogenetic trees based on the nucleotide sequences of the four gene fragments showed different topologies and were incongruent. The virulence-associated markers were distributed over the dendrograms and no association was found with phylogenetic clusters or clinical manifestations (chronic gastritis, gastric or duodenal ulcer, MALT lymphoma). Moreover, the ratios (calculated with the homoplasy test) ranged from 0742 to 0799, depending on the gene fragment examined. All these observations suggest that exists as a recombinant population. The clustering of the strains according to their geographical origin (USA/Europe, East Asia, South Africa) that has recently been demonstrated elsewhere could only be confirmed for the East Asian s1c strains. In contrast, the South African strains clustered together only in the tree. Presumably, recombination at the different loci has masked the evolutionary relationship among the strains.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): antibiotic resistance , H. pylori , housekeeping genes and MALT lymphoma, gastric mucosa-associated lymphoid tissue lymphoma
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2001-06-01
2025-06-19
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/content/journal/micro/10.1099/00221287-147-6-1693
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Population genetics of Helicobacter pylori in the southern part of Switzerland analysed by sequencing of four housekeeping genes (atpD, glnA, scoB and recA), and by vacA, cagA, iceA and IS605 genotypingThe GenBank accession numbers for the sequences reported in this paper are AY004351–AY004662
Microbiology 147, 1693 (2001); https://doi.org/10.1099/00221287-147-6-1693
/content/journal/micro/10.1099/00221287-147-6-1693
/content/journal/micro/10.1099/00221287-147-6-1693
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