1887

Abstract

Iron uptake, transport and storage in , the principal causative agent of human dental cavities, is unexplored despite early reports in the literature which predict a role for this trace metal in cariogenesis. Experiments in the authors’ laboratory revealed several iron-responsive proteins in , one of which reacted with a polyclonal antiserum directed against the FimA fimbrial adhesin from on Western blots. The results of Western blot and Northern hybridization experiments support an inverse relationship between iron availability and expression, and metal ion uptake experiments implicate FimA in Fe transport. Cloning of the homologue facilitated the construction of a knockout mutant which grew poorly in an iron-limiting medium relative to the wild-type progenitor strain, lending further support to a role for FimA in iron transport. The authors also identified and cloned a -like gene () located downstream of on the chromosome, and noted increased expression in a knockout mutant relative to wild-type on RNA spot blots and Western blots. The uptake of Fe, which was also significantly increased in this mutant, was compromised in a / double knockout. These findings are consistent with a role for Dlg in the iron-mediated regulation of , and possibly other iron transporters. Finally, the cariogenic potential of the and knockout mutants was not significantly different from that of the wild-type progenitor in a germ-free rat model.

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2001-06-01
2024-03-29
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