1887

Abstract

Plasmids of the incompatibility groups HI and HII (IncH plasmids) generally confer multiple antibiotic resistances upon their host pathogenic strain. IncHI group plasmids are distinguished by their property of optimal transfer by conjugation at temperatures below 30 °C, allowing for the spread of multiple antibiotic resistance outside their host natural environment, the gut. Plasmids of the IncHI1 subgroup encode multiple replicons. In the present study it is shown that the prototype IncHI2 subgroup plasmid, R478, contains at least two iteron-controlled autoreplicative regions, RepHI2A and RepHI1A. The DNA sequence and the molecular characteristics of each replicon region are described. RepHI2A is unique to plasmids of the IncHI2 subgroup and contains an unusually large number of iteron sequences downstream of the replication initiator gene. The nucleotide sequence of the replication initiator gene and of the iterons within RepHI1A show very close similarity with those of the previously reported RepHI1A replicon of the IncHI1 subgroup plasmid R27. The presence of RepHI1A on R478 most likely accounts for the observed incompatibility between R478 and plasmids of the IncHI1 subgroup. These are the first autoreplicative regions from an IncHI2 subgroup plasmid to be described.

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2001-06-01
2020-03-29
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. 1990; Basic local alignment search tool. J Mol Biol215:403–410[CrossRef]
    [Google Scholar]
  2. Anderson E. S.. 1975; The problem and implications of chloramphenicol resistance in the typhoid bacillus . . J Hyg74:289–299[CrossRef]
    [Google Scholar]
  3. Bachmann B. J.. 1972; Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev36:525–557
    [Google Scholar]
  4. Bethesda Research Laboratories 1986; BRL pUC host: E. coli DH5α competent cells. Bethesda Res Lab Focus8:29
    [Google Scholar]
  5. Birnboim H. C., Doly J.. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res7:1513–1523[CrossRef]
    [Google Scholar]
  6. Bradley D. E., Hughes V. M., Richards H., Datta N.. 1982; R plasmids of a new incompatibility group determine constitutive production of H pili. Plasmid7:230–238[CrossRef]
    [Google Scholar]
  7. Cordano A. M., Virgilio R.. 1996; Evolution of drug resistance in Salmonella panama isolates in Chile. Antimicrob Agents Chemother40:336–341
    [Google Scholar]
  8. Couturier M., Bex F., Bergquist P. L., Maas W. K.. 1988; Identification and classification of bacterial plasmids. Microbiol Rev52:375–395
    [Google Scholar]
  9. de Delucia P., Cairns J.. 1969; Isolation of an E.coli strain with a mutation affecting DNA polymerase. Nature224:1164–1167[CrossRef]
    [Google Scholar]
  10. Dower W. J., Millar J. F., Ragsdale C. W.. 1988; High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res16:6127–6145[CrossRef]
    [Google Scholar]
  11. Fellay R., Frey J., Krisch H.. 1987; Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene52:147–154[CrossRef]
    [Google Scholar]
  12. Fuller R. A., Kornberg A.. 1983; Purified DnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication. Proc Natl Acad Sci USA80:5817–5821[CrossRef]
    [Google Scholar]
  13. Gabant P., Chadi A. O., Couturier M.. 1994; Nucleotide sequence and replication characteristics of RepHI1B: a replicon specific to the IncHI1 plasmids. Plasmid31:111–120[CrossRef]
    [Google Scholar]
  14. Gardner R. C., Lane D., Bergquist P.. 1980; Replication mutants of the F-plasmid of Escherichia coli . II. Cloned replication regions of temperature sensitive mutants. Plasmid3:179–192[CrossRef]
    [Google Scholar]
  15. Geiger G. E., Modrich P.. 1979; Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of DpnI endonuclease. J Biol Chem254:1408–1413
    [Google Scholar]
  16. Goldstein F. W., Chumpitaz J. C., Guevara J. M., Papadopoulou B., Acar J. F., Vieu J. F.. 1986; Plasmid-mediated resistance to multiple antibiotics in Salmonella typhi . J Infect Dis153:261–266[CrossRef]
    [Google Scholar]
  17. Gosti-Testu F., Norris V., Brevet J.. 1983; Restriction map of Tn 7 . Plasmid10:96–99[CrossRef]
    [Google Scholar]
  18. Grindley N. D. F., Humphreys G. O., Anderson E. S.. 1973; Molecular studies of R factor incompatibility groups. Mol Gen Genet119:287–297
    [Google Scholar]
  19. Helinski D. R., Toukdarian A. E., Novick R. P.. others 1996; Replication control and other stable maintenance mechanisms of plasmids. In Escherichia coli and Salmonella: Cellular and Molecular Biology , 2nd edn. pp2295–2324 Edited by Neidhardt F. C.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Lane D., Gardner R. C.. 1979; Second Eco RI fragment of F capable of self-replication. J Bacteriol139:141–151
    [Google Scholar]
  21. Maher D., Colleran E.. 1987; The environmental significance of thermosensitive plasmids of the H incompatibility group. In Bioenvironmental systems pp1–36 Edited by Wise D. L.. Boca Raton, FL: CRC Press;
    [Google Scholar]
  22. Newnham P. J., Taylor D. E.. 1990; Genetic analysis of the transfer and incompatibility functions within the IncHI1 plasmid R27. Plasmid27:107–118
    [Google Scholar]
  23. Newnham P. J., Taylor D. E.. 1994; Molecular analysis of RepHI1A, a minimal replicon of the IncHI1 plasmid R27. Mol Microbiol11:757–768[CrossRef]
    [Google Scholar]
  24. Page D. T., Whelan K. F., Colleran E.. 1999; Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478 . . FEMS Microbiol Lett178:21–29
    [Google Scholar]
  25. Rodriguez-Lemoine V., Jacob A. E., Hedges R. W., Datta N.. 1975; Thermosensitive production of their transfer systems by group S plasmids . . J Gen Microbiol86:111–114[CrossRef]
    [Google Scholar]
  26. Roussel A. F., Chabbert Y.. 1978; Taxonomy and epidemiology of Gram-negative bacterial plasmids studied by DNA–DNA hybridization in formamide. J Gen Microbiol104:269–276[CrossRef]
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74:5463–5467[CrossRef]
    [Google Scholar]
  29. Saul D., Lane D., Bergquist P. L.. 1988; A replication region of the IncHI plasmid, R27, is highly homologous with the RepFIA replicon of F. Mol Microbiol2:219–225[CrossRef]
    [Google Scholar]
  30. Sobecky P. A., Mincer T. J., Chang M. C., Helinski D. R.. 1997; Plasmids isolated from marine sediment microbial communities contain replication and incompatibility regions unrelated to those of known plasmid groups. Appl Environ Microbiol63:888–895
    [Google Scholar]
  31. del Solar G., Giraldo R., Ruiz-Echevarria M. J., Espinosa M., Diaz-Orejas R.. 1998; Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev62:434–464
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J.. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-sensitive gap penalties and weight matrix choice. Nucleic Acids Res22:4673–4680[CrossRef]
    [Google Scholar]
  33. Vieira J., Messing J.. 1991; New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene100:189–194[CrossRef]
    [Google Scholar]
  34. Whelan K. F.. 1992; Genetic analysis of the IncHI2 incompatibility group plasmid R478 PhD thesis, University College Galway; Ireland:
    [Google Scholar]
  35. Whelan K. F., Colleran E.. 1992; Restriction endonuclease mapping of the HI2 incompatibility group plasmid, R478. J Bacteriol174:1197–1204
    [Google Scholar]
  36. Whiteley M., Taylor D. E.. 1983; Identification of DNA homologies among H incompatibility group plasmids by restriction enzyme digestion and Southern transfer hybridisation. Antimicrob Agents Chemother24:194–200[CrossRef]
    [Google Scholar]
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