A peptidorhamnomannan from the mycelium of is a potential diagnostic antigen of this emerging human pathogen Free

Abstract

The ascomycete is an emerging human pathogen frequently found in soil and polluted water. A peptidopolysaccharide antigen has been isolated from mycelial forms of , and characterized using chemical and immunological methods. Monosaccharide composition, methylation analysis, and H- and C-NMR spectra indicated the presence of a rhamnomannan with a structure distinct from those of similar components isolated from other fungi, containing Rha(1→3)Rha epitopes on side chains which may be linked (1→3) to (1→6)-linked mannose. The peptidorhamnomannan from reacted poorly with an antiserum raised against whole cells of and strongly with one against hyphae. These characteristics and immunological differences suggest that this major rhamnose-containing antigen of may be useful for the specific diagnosis of infections attributable to this fungus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-6-1499
2001-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/6/1471499a.html?itemId=/content/journal/micro/10.1099/00221287-147-6-1499&mimeType=html&fmt=ahah

References

  1. Ames B. N. 1966; Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118
    [Google Scholar]
  2. Belcher R. A., Nutten A. J., Sambrook C. M. 1954; The determination of glucosamine. Analyst 79:201–208 [CrossRef]
    [Google Scholar]
  3. Berenguer J., Diaz-Mediavilla J., Urra D., Muñoz P. 1989; Central nervous system infection caused by P. boydii : case report and review. Rev Infect Dis 11:890–896 [CrossRef]
    [Google Scholar]
  4. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Swith E. 1956; Colorimetric method for determination of sugar and related substances. Anal Chem 28:350–356 [CrossRef]
    [Google Scholar]
  5. Gorin P. A. J. 1981; Carbon-13 nuclear magnetic resonance spectroscopy of polysaccharides. Adv Carbohydr Chem Biochem 38:13–104
    [Google Scholar]
  6. Gorin P. A. J., Spencer J. F. T. 1970; Structures of the l- rhamno d-mannan from Ceratocystis ulmi and the d-gluco-d-mannan from Ceratocystis brunnea. . Carbohydr Res 13:339–349 [CrossRef]
    [Google Scholar]
  7. Haido R. M. T., Silva M. H., Ejzemberg R., Leitao E. A., Hearn V. M., Evans E. G. V., Barreto Bergter E. 1998; Analysis of peptidogalactomannan from the mycelial surface of Aspergillus fumigatus. . J Med Mycol 36:313–321 [CrossRef]
    [Google Scholar]
  8. Jansson P. E., Kenne L., Liedgren H., Lindberg B., Longen J. 1976; A practical guide to the methylation analysis of carbohydrates. Chem Commun Univ Stockholm 8.
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  10. Lake F. R., Tribe A. E., McAleer R., Frondist J., Thompson P. J. 1990; Mixed allergic bronchopulmonary fungal disease due to Pseudallescheria boydii and Aspergillus. Thorax 45489–492 [CrossRef]
    [Google Scholar]
  11. Lima O. C., Lopes-Bezerra L. M. 1997; Identification of a Concanavalin A-binding antigen of the cell surface of Sporothrix schenckii. . J Med Vet Mycol 35:167–172 [CrossRef]
    [Google Scholar]
  12. Lloyd K. O. 1970; Isolation, characterization and partial structure of peptidogalactomannan from the yeast form of Cladosporium wernecki. . Biochemistry 9:3446–3453 [CrossRef]
    [Google Scholar]
  13. Lloyd K. O., Travassos L. R. 1975; Immunochemical studies on l-rhamno-d-mannans of Sporothrix schenckii and related fungi by use of rabbit and human antisera. Carbohydr Res 40:89–97 [CrossRef]
    [Google Scholar]
  14. Lopes-Alves L., Travassos L. R., Previato J. O., Mendonça-Previato L. 1994; Novel antigenic determinants from peptidorhamnomannans of Sporothrix schenckii. Glycobiology 4281–288 [CrossRef]
    [Google Scholar]
  15. Lupan D. M., Cazin J. Jr 1976; Serological diagnosis of petrillidiosis (allescheriosis). I. Isolation and characterization of soluble antigens from Allescheria boydii and Monosporium apiospermum. . Mycopathologia 58:31–38 [CrossRef]
    [Google Scholar]
  16. Mendonça L., Gorin P. A. J., Lloyd K. O., Travassos L. R. 1976; Polymorphism of S. schenckii surface polysaccharides as a function of morphological differentiation. Biochemistry 15:2423–2431 [CrossRef]
    [Google Scholar]
  17. Ovodov Y. S., Evtushenko E. V., Vaskovsky V. E., Ovodova R. G., Soloveva T. F. 1967; Thin layer chromatography of carbohydrates. J Chromatogr 26:111–115 [CrossRef]
    [Google Scholar]
  18. Parente J. P., Cardon P., Leroy Y., Montreuil J., Fournet B., Ricart J. 1985; A convenient method for methylation of glycoprotein glycans in small amounts by using lithium methyl-sulfinyl-carbanion. Carbohydr Res 141:41–47 [CrossRef]
    [Google Scholar]
  19. Penha C. V. L., Lopes-Bezerra L. M. 2000; Concanavalin A-binding antigens of Sporothrix schenckii : a serological study. Med Mycol 38:1–7 [CrossRef]
    [Google Scholar]
  20. Rippon J. W. 1998; Pseudallescheriasis. Medical Mycology 651–680 Philadelphia: W. B. Saunders;
    [Google Scholar]
  21. Sawardeker J. S., Sloneker J. H., Jeanes H. 1965; Quantitative determination of monosaccharides as their acetates by gas-liquid chromatography. Anal Chem 37:1602–1604 [CrossRef]
    [Google Scholar]
  22. Spencer J. T. F., Gorin P. A. J. 1971; Systematics of the genera Ceratocystis and Graphium . Proton magnetic resonance spectra of the mannose-containing polysaccharides as an aid in classification. Mycologia 63:387–402 [CrossRef]
    [Google Scholar]
  23. Travassos L. R., Lloyd K. 1980; Sporothrix schenckii and related species of Ceratocystis. . Microbiol Rev 44:683–721
    [Google Scholar]
  24. Travassos L. R., Gorin P. A. J., Lloyd K. O. 1973; Comparison of the rhamnomannans from the human pathogen Sporothrix schenckii with those from the Ceratocystis species. Infect Immun 8:685–693
    [Google Scholar]
  25. Travassos L. R., Gorin P. A. J., Lloyd K. O. 1974; Discrimination between Sporothrix schenckii and Ceratocystis stenoceras rhamnomannans by proton and carbon-13 magnetic resonance spectroscopy. Infect Immun 9:674–680
    [Google Scholar]
  26. Voller A., Bidwell D., Bartlett A. 1976; Enzyme-linked immunosorbent assay. In Manual of Clinical Immunology pp 506–512 Edited by Rose N. R. Freidman H. M. Washington, DC: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-6-1499
Loading
/content/journal/micro/10.1099/00221287-147-6-1499
Loading

Data & Media loading...

Most cited Most Cited RSS feed