1887

Abstract

Despite its possible role in virulence, there has been little molecular characterization of members of the S-layer protein family of the group. It is hypothesized that the components of the S-layers are likely to display similar anchoring structures in and . Based on inferred sequence similarities, a DNA fragment encoding the cell-wall-anchoring domain of an S-layer component of subsp. NRRL 4045 was isolated. The complete gene was identified and sequenced. An ORF of 2463 nt was identified, which was predicted to encode a protein of 821 aa, SlpA. The mature SlpA protein (792 residues) carries three S-layer homology (SLH) motifs towards its amino terminus, each about 50 aa long. These motifs were sufficient to bind and cell walls by interacting with peptidoglycan-associated polymers, confirming a common wall-anchoring mechanism. The null-allele mutant was constructed and shown to possess no other abundant surface protein. It is proposed that the method described in this paper could be applied to the identification and deletion of any S-layer gene and is of great value for the molecular and functional characterization of these genes.

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2001-05-01
2019-12-15
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