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Volume 147, Issue 5

Molecular characterization of KT2440 gene regulation

The GenBank accession number for the sequence corresponding to the KT2440 chromosomal DNA fragment (2891 bp) cloned in pSH27, including the gene, is AF157048.

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Abstract

The gene of KT2440 encoding the heat-shock sigma factor σ was cloned and sequenced, and the translated gene product was predicted to be a protein of 325 kDa. The unambiguous role of the gene as a sigma factor was confirmed because the cloned gene complemented the growth defect, at 37 and 42 °C, of an mutant strain. Primer extension analysis showed that in the gene is expressed from three promoters in cells growing at 30 °C. Two of them, P1 and P3, share homology with the σ-dependent promoters, while the third one, P2, shows a typical σ-consensus sequence. The pattern of transcription initiation of the gene did not change in response to different stresses, i.e. a sudden heat shock or the addition of aromatic compounds. However, the predicted secondary structure of the 5′ region of the mRNA derived from the three different promoters suggests regulation at the level of translation efficiency and/or mRNA half-life. An inverted repeat sequence located 20 bp downstream of the stop codon was shown to function as a terminator in growing at temperatures from 18 to 42 °C.

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Keyword(s): aromatic hydrocarbons , DEPC, diethyl pyrocarbonate , downstream box , DS box, downstream box , heat-shock response , HS response, heat-shock response , stress response and σ32
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2001-05-01
2024-04-23
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Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulationThe GenBank accession number for the sequence corresponding to the P. putida KT2440 chromosomal DNA fragment (2891 bp) cloned in pSH27, including the rpoH gene, is AF157048.
Microbiology 147, 1323 (2001); https://doi.org/10.1099/00221287-147-5-1323
/content/journal/micro/10.1099/00221287-147-5-1323
/content/journal/micro/10.1099/00221287-147-5-1323
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