1887

Abstract

Expression of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is developmentally regulated, and induced by glucose in . A gene, , encoding a protein similar to the AraC/XylS family of bacterial transcriptional regulators was identified upstream of . The gene was constitutively expressed from a single promoter during the course of differentiation. By integrative transformation, via double crossover, a stable null mutant of the gene was obtained. The mutation only slightly affected growth, and had no effect on differentiation of . However, transcription of the GAPDH-encoding gene was substantially reduced in the Δ null mutant, irrespective of carbon source used. Though GAPDH activity was about 15-fold lower in the mutant, the substantial enzyme activity remained, suggesting the presence of a second GAPDH which is sufficient to ensure growth. The GapR protein, overproduced in , was shown to bind upstream of the -P promoter region. The results indicate a direct role of GapR in regulation of expression in

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2001-05-01
2020-01-23
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