: a Zn-responsive regulatory element of

The GenBank accession number for the sequence reported in this paper is AF101263.

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Abstract

A putative operon encoding a probable zinc-responsive regulatory element () and components of an ABC-type transporter () have been characterized in . The gene was inactivated but apparently this did not alter Zn uptake. Expression of is at a low level under a range of ion conditions. To allow inducible expression of the operon, a construct was made placing it under the control of the IPTG-inducible P promoter. Using this approach, it was shown that is able to repress expression of the entire operon in a Zn-dependent manner, and that and are likely to be involved in high-affinity ion uptake. has no apparent role in pathogenicity in a lesion model of infection.

Keyword(s): fur , metals , regulation , transcription and zinc
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2001-05-01
2024-03-28
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References

  1. Achenbach L. A., Yang W. 1997; The fur gene from Klebsiella pneumoniae : characterization, genomic organization and phylogenetic analysis. Gene 185:201–207 [CrossRef]
    [Google Scholar]
  2. Althaus E. W., Outten C. E., Olson K. E., Cao H., O’Halloran T. V. 1999; The ferric uptake regulation (Fur) repressor is a zinc metalloprotein. Biochemistry 38:6559–6569 [CrossRef]
    [Google Scholar]
  3. Bearden S. W., Perry R. D. 1999; The Yfe system of Yersinia pestis transports iron and manganese and is required for full virulence of plague. Mol Microbiol 32:403–414 [CrossRef]
    [Google Scholar]
  4. Bsat N., Herbig A., Casillas-Martinez L., Setlow P., Helmann J. D. 1998; Bacillus subtilis contains multiple Fur homologues: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors. Mol Microbiol 29:189–198 [CrossRef]
    [Google Scholar]
  5. Chan P. F., Foster S. J. 1998; The role of environmental factors in the regulation of virulence-determinant expression in Staphylococcus aureus 8325-4. Microbiology 144:2469–2479 [CrossRef]
    [Google Scholar]
  6. Chan P. F., Foster S. J., Ingham E., Clements M. O. 1998; The Staphylococcus aureus alternative sigma factor sigmaB controls the environmental stress response but not starvation survival or pathogenicity in a mouse abscess model. J Bacteriol 180:6082–6089
    [Google Scholar]
  7. Clements M. O., Watson S. P., Foster S. J. 1999; Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity. J Bacteriol 181:3898–3903
    [Google Scholar]
  8. Cockayne A., Hill P. J., Powell N. B., Bishop K., Sims C., Williams P. 1998; Molecular cloning of a 32-kilodalton lipoprotein component of a novel iron-regulated Staphylococcus epidermidis ABC transporter. Infect Immun 66:3767–3774
    [Google Scholar]
  9. Cornelissen C. N., Kelley M., Hobbs M. M., Anderson J. E., Cannon J. G., Cohen M. S., Sparling P. F. 1998; The transferrin receptor expressed by gonococcal strain FA1090 is required for the experimental infection of human male volunteers. Mol Microbiol 27:611–616 [CrossRef]
    [Google Scholar]
  10. Courcol R. J., Trivier D., Bissinger M. C., Martin G. R., Brown M. R. 1997; Siderophore production by Staphylococcus aureus and identification of iron-regulated proteins. Infect Immun 65:1944–1948
    [Google Scholar]
  11. Dalet K., Gouin E., Cenatiempo Y., Cossart P., Hechard Y. 1999; Characterisation of a new operon encoding a Zur-like protein and an associated ABC zinc permease in Listeria monocytogenes . FEMS Microbiol Lett 174:111–116 [CrossRef]
    [Google Scholar]
  12. Dawson-Saunders B., Trapp R. G. 1990 Basic and Clinical Biostatistics Norwalk, CT: Appleton & Lange;
    [Google Scholar]
  13. Dintilhac A., Alloing G., Granadel C., Claverys J. P. 1997; Competence and virulence of Streptococcus pneumoniae : Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol Microbiol 25:727–739 [CrossRef]
    [Google Scholar]
  14. Domingue P. A., Lambert P. A., Brown M. R. 1989; Iron depletion alters surface-associated properties of Staphylococcus aureus and its association to human neutrophils in chemiluminescence. FEMS Microbiol Lett 50:265–268
    [Google Scholar]
  15. Driessen C., Hirv K., Kirchner H., Rink L. 1995a; Divergent effects of zinc on different bacterial pathogenic agents. J Infect Dis 171:486–489 [CrossRef]
    [Google Scholar]
  16. Driessen C., Hirv K., Kirchner H., Rink L. 1995b; Zinc regulates cytokine induction by superantigens and lipopolysaccharide. Immunology 84:272–277
    [Google Scholar]
  17. Escolar L., Perez-Martin J., de Lorenzo V. 1999; Opening the iron box: transcriptional metalloregulation by the Fur protein. J Bacteriol 181:6223–6229
    [Google Scholar]
  18. Foster S. J. 1991; Cloning, expression, sequence analysis and biochemical characterization of an autolytic amidase of Bacillus subtilis 168 trpC2 . J Gen Microbiol 137:1987–1998 [CrossRef]
    [Google Scholar]
  19. Gaballa A., Helmann J. D. 1998; Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis . J Bacteriol 180:5815–5821
    [Google Scholar]
  20. Guerout-Fleury A. M., Shazand K., Frandsen N., Stragier P. 1995; Antibiotic-resistance cassettes for Bacillus subtilis . Gene 167:335–336 [CrossRef]
    [Google Scholar]
  21. Haag H., Fiedler H. P., Meiwes J., Drechsel H., Jung G., Zahner H. 1994; Isolation and biological characterization of staphyloferrin B, a compound with siderophore activity from staphylococci. FEMS Microbiol Lett 115:125–130 [CrossRef]
    [Google Scholar]
  22. Heidrich C., Hantke K., Bierbaum G., Sahl H. G. 1996; Identification and analysis of a gene encoding a Fur-like protein of Staphylococcus epidermidis . FEMS Microbiol Lett 140:253–259 [CrossRef]
    [Google Scholar]
  23. Heinrichs J. H., Gatlin L. E., Kunsch C., Choi G. H., Hanson M. S. 1999; Identification and characterization of SirA, an iron-regulated protein from Staphylococcus aureus . J Bacteriol 181:1436–1443
    [Google Scholar]
  24. Horsburgh M. J., Ingham E., Foster S. J. 2001; In Staphylococcus aureus , Fur is an interactive regulator with PerR, contributes to virulence, and is necessary for oxidative stress resistance through positive regulation of catalase and iron homeostasis. J Bacteriol 183:468–475 [CrossRef]
    [Google Scholar]
  25. Janakiraman A., Slauch J. M. 2000; The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium . Mol Microbiol 35:1146–1155 [CrossRef]
    [Google Scholar]
  26. Janulczyk R., Pallon J., Bjorck L. 1999; Identification and characterization of a Streptococcus pyogenes ABC transporter with multiple specificity for metal cations. Mol Microbiol 34:596–606 [CrossRef]
    [Google Scholar]
  27. Kreiswirth B. N., Lofdahl S., Betley M. J., O’Reilly M., Schlievert P. M., Bergdoll M. S., Novick R. P. 1983; The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709–712 [CrossRef]
    [Google Scholar]
  28. Levin J. D., Shapiro R., Demple B. 1991; Metalloenzymes in DNA repair. Escherichia coli endonuclease IV and Saccharomyces cerevisiae Apn1. J Biol Chem 266:22893–22898
    [Google Scholar]
  29. Lewis D. A., Klesney-Tait J., Lumbley S. R., Ward C. K., Latimer J. L., Ison C. A., Hansen E. J. 1999; Identification of the znuA -encoded periplasmic zinc transport protein of Haemophilus ducreyi . Infect Immun 67:5060–5068
    [Google Scholar]
  30. Lindsay J. A., Riley T. V. 1994; Staphylococcal iron requirements, siderophore production, and iron-regulated protein expression. Infect Immun 62:2309–2314
    [Google Scholar]
  31. Lindsay J. A., Riley T. V., Mee B. J. 1995; Staphylococcus aureus but not Staphylococcus epidermidis can acquire iron from transferrin. Microbiology 141:197–203 [CrossRef]
    [Google Scholar]
  32. Lindsay J. A., Ruzin A., Ross H. F., Kurepina N., Novick R. P. 1998; The gene for toxic shock toxin is carried by a family of mobile pathogenicity islands in Staphylococcus aureus . Mol Microbiol 29:527–543 [CrossRef]
    [Google Scholar]
  33. Litwin C. M., Calderwood S. B. 1993; Role of iron in regulation of virulence genes. Clin Microbiol Rev 6:137–149
    [Google Scholar]
  34. Magneson G. R., Puvathingal J. M., Ray W. J. Jr 1987; The concentrations of free Mg2+ and free Zn2+ in equine blood plasma. J Biol Chem 262:11140–11148
    [Google Scholar]
  35. Meiwes J., Fiedler H. P., Haag H., Zahner H., Konetschny-Rapp S., Jung G. 1990; Isolation and characterization of staphyloferrin A, a compound with siderophore activity from Staphylococcus hyicus DSM 20459. FEMS Microbiol Lett 55:201–205
    [Google Scholar]
  36. Modun B., Williams P. 1999; The staphylococcal transferrin-binding protein is a cell wall glyceraldehyde-3-phosphate dehydrogenase. Infect Immun 67:1086–1092
    [Google Scholar]
  37. Modun B., Kendall D., Williams P. 1994; Staphylococci express a receptor for human transferrin: identification of a 42-kilodalton cell wall transferrin-binding protein. Infect Immun 62:3850–3858
    [Google Scholar]
  38. Modun B., Evans R. W., Joannou C. L., Williams P. 1998; Receptor-mediated recognition and uptake of iron from human transferrin by Staphylococcus aureus and Staphylococcus epidermidis . Infect Immun 66:3591–3596
    [Google Scholar]
  39. Novak R., Braun J. S., Charpentier E., Tuomanen E. 1998; Penicillin tolerance genes of Streptococcus pneumoniae : the ABC-type manganese permease complex Psa. Mol Microbiol 29:1285–1296 [CrossRef]
    [Google Scholar]
  40. Novick R. 1967; Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus . Virology 33:155–166 [CrossRef]
    [Google Scholar]
  41. Novick R. P. 1991; Genetic systems in staphylococci. Methods Enzymol 204:587–636
    [Google Scholar]
  42. Patzer S. I., Hantke K. 1998; The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli . Mol Microbiol 28:1199–1210 [CrossRef]
    [Google Scholar]
  43. Que Q., Helmann J. D. 2000; Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins. Mol Microbiol 35:1454–1468
    [Google Scholar]
  44. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  45. Schenk S., Laddaga R. A. 1992; Improved method for electroporation of Staphylococcus aureus . FEMS Microbiol Lett 73:133–138
    [Google Scholar]
  46. Trivier D., Davril M., Houdret N., Courcol R. J. 1995; Influence of iron depletion on growth kinetics, siderophore production, and protein expression of Staphylococcus aureus . FEMS Microbiol Lett 127:195–199 [CrossRef]
    [Google Scholar]
  47. Vagner V., Dervyn E., Ehrlich S. D. 1998; A vector for systematic gene inactivation in Bacillus subtilis . Microbiology 144:3097–3104 [CrossRef]
    [Google Scholar]
  48. Watson S. P., Clements M. O., Foster S. J. 1998; Characterization of the starvation-survival response of Staphylococcus aureus . J Bacteriol 180:1750–1758
    [Google Scholar]
  49. Xiong A., Jayaswal R. K. 1998; Molecular characterization of a chromosomal determinant conferring resistance to zinc and cobalt ions in Staphylococcus aureus . J Bacteriol 180:4024–4029
    [Google Scholar]
  50. Xiong A., Singh V. K., Cabrera G., Jayaswal R. K. 2000; Molecular characterization of the ferric-uptake regulator, fur , from Staphylococcus aureus . Microbiology 146:659–668
    [Google Scholar]
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