1887

Abstract

The study of gene regulation in many organisms has been facilitated by the development of reporter genes. The authors report the use of from , a gene encoding a β-galactosidase, as a reporter for the fungal pathogen . As test cases, was placed under control of three different promoters: (maltase), inducible by maltose; (hyphal cell wall protein), induced by conditions that promote filamentous growth; and (actin). These constructs were each integrated into the genome and β-galactosidase activity was readily detected from these strains, but only under the appropriate growth conditions. β-Galactosidase activity could be detected by several methods: quantitative liquid assays using permeabilized cells, colorimetric assays of colonies replicated to paper filters, and coloration of colonies growing on medium containing the indicator X-Gal. These results show the usefulness of as a monitor of gene regulation in this medically important yeast.

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2001-05-01
2022-01-17
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