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Volume 147, Issue 5

mutations in and quorum sensing systems result in milder chronic lung infection Free

Abstract

To understand the importance of quorum sensing in chronic lung infection, the pathogenic effects of the wild-type PAO1 and its double mutant, PAO1 , in which the signal-generating parts of the quorum sensing systems are defective were compared. The rat model of lung infection was used in the present study. The rats were killed on days 3, 7, 14 and 28 after infection with the strains. The results showed that during the early stages of infection, the PAO1 double mutant induced a stronger serum antibody response, higher production of pulmonary interferon γ, and more powerful blood polymorphonuclear leukocyte (PMN) chemiluminescence compared to its wild-type counterpart. On days 14 and 28 post-infection, significantly milder lung pathology, a reduction in the number of mast cells present in the lung foci, a reduced number of lung bacteria, and minor serum IgG and IgG1 responses but increased lung interferon γ production were detected in the group infected with the PAO1 double mutant when compared with the PAO1-infected group. Delayed immune responses were observed in the PAO1-infected group and they might be associated with the production of virulence factors that are controlled by the quorum sensing systems. The conclusion of this study is that functional and genes of PAO1 play a significant role during lung infection.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): AHL, N-acylhomoserine lactone , CF, cystic fibrosis , chronic lung infection , cystic fibrosis , IFN-γ, interferon γ , IL, interleukin , lasI and rhlI , LIMP, lung index of macroscopic pathology , MN, mononuclear leukocyte , N-acylhomoserine lactones , p.i., post-infection , PMN, polymorphonuclear leukocyte , rat model and St-Ag, P. aeruginosa standard antigen
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2001-05-01
2024-04-24
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Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection
Microbiology 147, 1105 (2001); https://doi.org/10.1099/00221287-147-5-1105
/content/journal/micro/10.1099/00221287-147-5-1105
/content/journal/micro/10.1099/00221287-147-5-1105
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