1887

Abstract

isolates from human sporadic and epidemic cases (=119) and from animal cases (=76) were characterized by automated ribotyping and PCR-restriction fragment length polymorphism (PCR-RFLP) typing of the virulence genes and . This combination of typing methods differentiated 39 distinctive strains, each reflecting a unique combination of ribotypes, and alleles. Simpson’s index of discrimination indicated a high discriminatory ability of ribotyping for both animal (0867) and human isolates (0857), which was further increased by the addition of and typing (0916 and 0904, respectively). Ribotype and allele data were further used to group isolates into three genetically distinct lineages. Each lineage is composed of several ribotype fragment subsets, each of which contains multiple ribotypes characterized by common ribotype fragments. To determine whether certain clones of show indications for unique pathogenic potential or host specificity, frequency distributions for five genetic characteristics (i.e. lineage, ribotype, ribotype fragment subset and and allele) were calculated for isolates from animal cases, human epidemic cases and human sporadic cases. Lineage III isolates were found less frequently in human cases (1 of 119 isolates) than in animal cases (8 of 76 isolates; =0003). These results suggest the possibility of host specificity for non-primate mammals among lineage III strains. In addition, lineage I strains were found more frequently among human cases than among animal cases (<0001). Among the eight alleles observed, allele 1 was more common among human isolates as compared to animal isolates (=0002). We also identified one ribotype (DUP-1030) which was significantly more common among animal isolates (=0005) and one ribotype (DUP-1038; lineage I) which was significantly more common among human epidemic isolates as compared to human sporadic isolates (<0001). These findings confirm the presence of clonal groups of , which appear to be characterized by unique virulence or host specificity patterns. This study also establishes baseline data describing the genetic diversity of human and animal isolates which can be utilized in future surveillance programmes to track the emergence of new strains.

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2001-05-01
2019-09-16
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References

  1. Bille, J. (1990). Epidemiology of human listeriosis in Europe, with special reference to Swiss outbreak. In Foodborne Listeriosis, pp. 71–74. Amsterdam: Elsevier.
  2. Bille, J. & Rocourt, J. ( 1996; ). WHO International Multicentre Listeria monocytogenes subtyping study: rationale and set-up of the study. Int J Food Microbiol 32, 251-262.[CrossRef]
    [Google Scholar]
  3. Boerlin, P. & Piffaretti, J.-C. ( 1991; ). Typing of human, food and environmental isolates of Listeria monocytogenes by multilocus enzyme electrophoresis. Appl Environ Microbiol 57, 1624-1629.
    [Google Scholar]
  4. Brosch, R., Chen, J. & Luchansky, J. B. ( 1994; ). Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar. Appl Environ Microbiol 60, 2584-2592.
    [Google Scholar]
  5. Bruce, J. ( 1996; ). Automated system rapidly identifies and characterizes micro-organisms in food. Food Technol 50, 77-81.
    [Google Scholar]
  6. Bruce, J. L., Hubner, R. J., Cole, E. M., McDowell, C. I. & Webster, J. A. ( 1995; ). Sets of EcoRI fragments containing rRNA sequences are conserved among different strains of Listeria monocytogenes. Proc Natl Acad Sci USA 92, 5229-5233.[CrossRef]
    [Google Scholar]
  7. Carbonelle, B., Cottin, J., Parvery, F., Chambreuil, G., Kouyoumdjian, S., Le Lirzin, M., Cordier, G. & Vincent, F. ( 1978; ). Epidemie de listeriose dans l’ouest de la France (1975–1976). Rev Epidemiol Sante Publique 26, 451–467.
    [Google Scholar]
  8. Caugant, D. A., Ashton, F. E., Bibb, W. F., Boerlin, P., Donachie, W., Low, C., Gilmour, A., Harvey, J. & Norrung, B. ( 1996; ). Multilocus enzyme electrophoresis for characterization of Listeria monocytogenes isolates: results of an international comparative study. Int J Food Microbiol 32, 301-310.[CrossRef]
    [Google Scholar]
  9. Chakraborty, T., Ebel, F., Wehland, J., Dufrenne, J. & Notermans, S. ( 1994; ). Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes. FEMS Immunol Med Microbiol 10, 1-10.[CrossRef]
    [Google Scholar]
  10. Davies, R. L., Arkinsaw, S. & Selander, R. K. ( 1997; ). Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep. Infect Immun 65, 3585-3593.
    [Google Scholar]
  11. Farber, J. M. & Peterkin, P. I. ( 1991; ). Listeria monocytogenes, a food-borne pathogen. Microbiol Rev 55, 476-511.
    [Google Scholar]
  12. Fleming, D. W., Cochi, S. L., MacDonald, K. L. & 7 other authors ( 1985; ). Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med 312, 404–407.[CrossRef]
    [Google Scholar]
  13. Graves, L. M., Swaminathan, B., Reeves, M. W., Hunter, S. B., Weaver, R. E., Plikaytis, B. D. & Schuchat, A. ( 1994; ). Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping Listeria monocytogenes isolates. J Clin Microbiol 32, 2936-2943.
    [Google Scholar]
  14. Gutekunst, K. A., Holloway, B. P. & Carlone, G. M. ( 1992; ). DNA sequence heterogeneity in the gene encoding a 60-kilodalton extracellular protein of Listeria monocytogenes and other Listeria species. Can J Microbiol 38, 865-870.[CrossRef]
    [Google Scholar]
  15. Helmig, R., Uldbjerg, N., Boris, J. & Kilian, M. ( 1993; ). Clonal analysis of Streptococcus agalactiae isolated from infants with neonatal sepsis or meningitis and their mothers and from healthy pregnant women. J Infect Dis 168, 904-909.[CrossRef]
    [Google Scholar]
  16. Ho, J. L., Shands, K. N., Friedland, G., Eckind, P. & Fraser, D. W. ( 1986; ). An outbreak of type 4b Listeria monocytogenes infection involving patients from eight Boston hospitals. Arch Intern Med 146, 520-524.[CrossRef]
    [Google Scholar]
  17. Hof, H. & Rocourt, J. ( 1992; ). Is any strain of Listeria monocytogenes detected in food a health risk? Int J Food Microbiol 16, 173-182.[CrossRef]
    [Google Scholar]
  18. Hunter, P. & Gaston, M. A. ( 1988; ). Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol 26, 2465-2466.
    [Google Scholar]
  19. Kapur, V., Sischo, W. M., Greer, R. S., Whittam, T. & Musser, J. M. ( 1995; ). Molecular population genetic analysis of Staphylococcus aureus recovered from cows. J Clin Microbiol 33, 376-380.
    [Google Scholar]
  20. Lee, P. K., Kreiswirth, B. N., Deringer, J. R., Projan, S. J., Eisner, W., Smith, B. L., Carlson, E., Novick, R. P. & Schlievert, P. M. ( 1992; ). Nucleotide sequence and biological properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus. J Infect Dis 165, 1056-1063.[CrossRef]
    [Google Scholar]
  21. Linnan, M. J., Mascola, L., Lou, X. D. & 12 other authors ( 1988; ). Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med 319, 823–828.[CrossRef]
    [Google Scholar]
  22. Louie, M., Jayaratne, P., Luchsinger, I., Devenish, J., Yao, J., Schlech, W. & Simor, I. ( 1996; ). Comparison of ribotyping, arbitrarily primed PCR and pulsed-field gel electrophoresis for molecular typing of Listeria monocytogenes. J Clin Micro 34, 15-19.
    [Google Scholar]
  23. Marr, J. C., Lyon, J. D., Roberson, J. R., Lupher, M., Davies, W. C. & Bohach, G. A. ( 1993; ). Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications. Infect Immun 61, 4254-4262.
    [Google Scholar]
  24. McLauchlin, J. ( 1990; ). Distribution of serovars of Listeria monocytogenes isolated from different categories of patients with listeriosis. Eur J Clin Microbiol Infect Dis 9, 210-213.[CrossRef]
    [Google Scholar]
  25. McLauchlin, J., Audurier, A. & Taylor, A. G. ( 1986; ). Aspects of the epidemiology of human Listeria monocytogenes infections in Britain 1967–1984; the use of serotyping and phage typing. J Med Microbiol 22, 367-377.[CrossRef]
    [Google Scholar]
  26. McLauchlin, J., Hall, S. M., Velani, S. K. & Gilbert, R. J. ( 1991; ). Human listeriosis and paté: a possible association. Br Med J 303, 773-775.[CrossRef]
    [Google Scholar]
  27. Mead, P., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., Griffin, P. M. & Tauxe, R. V. ( 1999; ). Food-related illness and death in the United States. Emerg Infect Dis 5, 607-625.[CrossRef]
    [Google Scholar]
  28. Musser, J. M. & Selander, R. K. ( 1990; ). Genetic analysis of natural populations of Staphylococcus aureus. In Molecular Biology of the Staphylococci , pp. 59-67. Edited by R. Novick & R. A. Skurray. New York:VCH.
  29. Musser, J. M., Bemis, D. A., Ishikawa, H. & Selander, R. K. ( 1987; ). Clonal diversity and host distribution in Bordetella bronchiseptica. J Bacteriol 169, 2793-2803.
    [Google Scholar]
  30. Musser, J. M., Mattingly, S. J., Quentin, R., Goudeau, A. & Selander, R. K. ( 1989; ). Identification of a high-virulence clone of type III Streptococcus agalactiae (group B streptococcus) causing invasive neonatal disease. Proc Natl Acad Sci USA 86, 4731-4735.[CrossRef]
    [Google Scholar]
  31. Piffaretti, J.-C., Kressebuch, H., Aeschenbacher, M., Bille, J., Bannerman, E., Musser, J. M., Selander, R. K. & Rocourt, J. ( 1989; ). Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. Proc Natl Acad Sci USA 86, 3818-3822.[CrossRef]
    [Google Scholar]
  32. Quentin, R., Goudeau, A., Wallace, R. J., Smith, A. L., Selander, R. K. & Musser, J. M. ( 1990; ). Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species. J Gen Microbiol 136, 1203-1209.[CrossRef]
    [Google Scholar]
  33. Quentin, R., Huet, H., Wang, F., Geslin, P., Goudeau, A. & Selander, R. ( 1995; ). Characterization of Streptococcus agalactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal disease. J Clin Microbiol 33, 2576-2581.
    [Google Scholar]
  34. Rasmussen, O. F., Beck, T., Olsen, J. E., Dons, L. & Rossen, L. ( 1991; ). Listeria monocytogenes isolates can be classified into two major types according to the sequence of the listeriolysin gene. Infect Immun 59, 3945-3951.
    [Google Scholar]
  35. Rasmussen, O. F., Skouboe, P., Dons, L., Rosen, L. & Olsen, J. E. ( 1995; ). Listeria monocytogenes exists in at least three evolutionary lines: evidence from flagellin, invasive associated protein and listeriolysin O genes. Microbiology 141, 2053-2061.[CrossRef]
    [Google Scholar]
  36. Schlech, W. F., Lavigne, P. M., Bortolussi, R. A. & 8 other authors ( 1983; ). Epidemic listeriosis: evidence for transmission by food. N Engl J Med 308, 203–206.[CrossRef]
    [Google Scholar]
  37. Schwartz, B., Hexter, D., Broome, C. V. & 7 other authors ( 1989; ). Investigation of an outbreak of listeriosis: new hypotheses for the aetiology of epidemic Listeria monocytogenes infections. J Infect Dis 159, 680–685.[CrossRef]
    [Google Scholar]
  38. Selander, R. K. & Musser, J. M. ( 1990; ). Population genetics of bacterial pathogenesis. In Molecular Basis of Bacterial Pathogenesis , pp. 11-36. Edited by B. H. Iglewski & V. L. Clark. San Diego, CA:Academic Press.
  39. Selander, R. K., Musser, J. M., Caugant, D. A., Gilmour, M. N. & Whittam, T. S. ( 1987; ). Population genetics of pathogenic bacteria. Microb Pathog 3, 1-7.[CrossRef]
    [Google Scholar]
  40. Swaminathan, B., Hunter, S. B., Desmarchelier, P. M. & 10 other authors ( 1996; ). WHO-sponsored international collaborative study to evaluate methods for subtyping Listeria monocytogenes: restriction fragment length polymorphism (RFLP) analysis using ribotyping and Southern blotting with two probes derived from L. monocytogenes chromosome. Int J Food Microbiol 32, 263–278.[CrossRef]
    [Google Scholar]
  41. Vines, A., Reeves, M. W., Hunter, S. & Swaminathan, B. ( 1992; ). Restriction fragment length polymorphism in four virulence-associated genes of Listeria monocytogenes. Res Microbiol 143, 281-294.[CrossRef]
    [Google Scholar]
  42. Wiedmann, M., Bruce, J. L., Keating, C., Johnson, A., McDonough, P. L. & Batt, C. A. ( 1997; ). Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect Immun 65, 2707-2716.
    [Google Scholar]
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