%0 Journal Article %A O’Leary, Niall D. %A O’Connor, Kevin E. %A Duetz, Wouter %A Dobson, Alan D. W. %T Transcriptional regulation of styrene degradation in Pseudomonas putida CA-3The GenBank accession number for the sequence determined in this work is AF257095. %D 2001 %J Microbiology, %V 147 %N 4 %P 973-979 %@ 1465-2080 %R https://doi.org/10.1099/00221287-147-4-973 %K induction %K SMO, styrene monooxygenase %K PAA, phenylacetic acid %K PACoA, phenylacetate–CoA %K styrene %K Pseudomonas putida %K catabolite repression %I Microbiology Society, %X The styrene degradative pathway in Pseudmonas putida CA-3 has previously been shown to be divided into an upper pathway involving the conversion of styrene to phenylacetic acid and a lower pathway for the subsequent degradation of phenylacetic acid. It is reported here that expression of the regulatory genes styS and styR is essential for transcription of the upper pathway, but not for degradation of the lower pathway inducer, phenylacetic acid. The presence of phenylacetic acid in the growth medium completely repressed the upper pathway enzymes even in the presence of styrene, the upper pathway inducer. This repression is mediated at the transcription level by preventing expression of the styS and styR regulatory genes. Finally, an examination was made of the various stages of the diauxic growth curve obtained when P. putida CA-3 was grown on styrene together with an additional carbon source and it is reported that catabolite repression may involve a different mechanism to transcriptional repression by an additional carbon source. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-4-973