1887

Abstract

Transcription of the region of the K38 chromosome was analysed by Northern RNA–DNA hybridization, RT-PCR and primer extension. Transcription of a dicistronic mRNA and a tetracistronic mRNA was induced by silver and was initiated from the sigma-70 promoters and . Expression of β-galactosidase activity from a Φ() operon fusion was also induced by Ag and Cu, but not by Zn. In-frame deletion of from the chromosome yielded a silver-sensitive mutant strain which did not differ in its copper resistance from its wild-type strain. On the other hand, deletion of the gene for the copper-exporting P-type ATPase CopA resulted in copper sensitivity, but not in silver sensitivity. A Δ Δ double mutant strain behaved towards copper as the Δ strain and towards silver as the Δ strain. Thus, in , the YlcBCD–YbdE system may be involved in silver- but not in copper resistance, and CopA may be involved in copper- but not in silver resistance.

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2001-04-01
2020-09-28
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