1887

Abstract

The promoter region consists of two divergent promoters, directing expression of the major chemotaxis operon and a novel gene (hemotaxis ssociated ene ). Analyses of start sites by primer extension and alignment of the divergent promoters revealed significant similarities between them at the −35 promoter region. Both and are differentially expressed in the cell cycle, with maximal activation of transcription in predivisional cells. The main difference between the and promoters is that, in common with the flagellin, is expressed in swarmer cells. A promoter fusion that contains 36 bases of untranslated mRNA has sufficient information to segregate the transcript to swarmer cells. Expression of and was dependent on DNA replication. Transcriptional epistasis experiments were performed to identify potential regulators in the flagellar hierarchy. The sigma factor RpoN, which is required for flagellar biogenesis, is not required for and expression. Mutations in the genes for the MS-ring and the switch complex (flagellar class II mutants) do not affect expression of and . However, CtrA, an essential response regulator of flagellar gene transcription, is required.

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2001-04-01
2020-01-19
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