High-resolution data of actively gliding wild-type bacteria of four different species and of four different gliding mutants of Myxococcus xanthus were obtained from scanning electron micrographs. By shock freezing and freeze drying, motility-associated surface patterns could be fixed and consequently distinct intermediate states of motion could be observed for the first time. It is shown that these topographic patterns are immediately lost when gliding motility is stopped by blocking the respiratory chain with potassium cyanide or sodium azide. From the surface topography, the mode of action of the gliding apparatus of all four bacterial species examined can be described as a twisted circularly closed ‘band’. During gliding, groups of nodes of the supertwisted apparatus show evidence of travelling like waves along the trichomes. However, the spacing between the nodes is not constant but varies within a certain range. This indicates that they are flexibly modulated as a consequence of the gliding state of the individual trichome.
AbbanatD. R.,
LeadbetterE. R.,
GodchauxW.III,
EscherA.
1986; Sulphonolipids are molecular determinants of gliding motility. Nature 324:367–369[CrossRef]
BeatsonP. J.,
MarshallK. C.
1994; A proposed helical mechanism for gliding motility in three gliding bacteria (order Cytophagales. Can J Microbiol 40:173–183[CrossRef]
CastenholzR. W.
1982; Motility and taxes. In The Biology of Cyanobacteria pp 413–439 Edited by
CarrN. G.,
WhittonB. A.
Oxford: Blackwell Scientific Publications;
DuxburryT.,
HumphreyB. A.,
MarshallK. C.
1980; Continuous observations of bacterial gliding motility in a dialysis microchamber: the effects of inhibitors. Arch Microbiol 124:169–175
Dzink-FoxJ. L.,
LeadbetterE. R.,
GodchauxW.III.
1997; Acetate acts as a protonophore and differentially affects bead movement and cell migration of the gliding bacterium Cytophaga johnsonae (Flavobacterium johnsoniae). Microbiology 143:3693–3701[CrossRef]
FreeseA.,
ReichenbachH.,
LünsdorfH. 1997; Further characterization and in situ localization of chain-like aggregates of the gliding bacteria Myxococcus fulvus and Myxococcus xanthus . J Bacteriol 179:1246–1252
GodwinS. L.,
FletcherM.,
BurchardR. T.
1989; Interference reflection microscopic study of sites of association between gliding bacteria and glass substrata. J Bacteriol 171:4589–4594
GorskiL.,
LeadbetterE. R.,
GodchauxW.III.
1991; Temporal sequence of trait recovery during phenotypic curing of a Cytophaga johnsonae motility mutant. J Bacteriol 173:7534–7539
GorskiL.,
GodchauxW.III,
LeadbetterE. R.,
WagnerR. R.
1992; Diversity in surface features of Cytophaga johnsonae motility mutants. J Gen Microbiol 138:1767–1772[CrossRef]
GorskiL.,
GodchauxW.III,
LeadbetterE. R.
1993; Structural specificity of sugars that inhibit gliding motility of Cytophaga johnsonae . Arch Microbiol 160:121–125[CrossRef]
HäderD. P.VogelK.
1991; Interactive image analysis system to determine the motility and velocity of cyanobacterial filaments. J Biochem Biophys Methods 22:289–300[CrossRef]
HodgkinJ.,
KaiserD.
1979a; Genetics of gliding motility in Myxococcus xanthus (Myxobacterales): genes controlling movement of single cells. Mol Gen Genet 171:167–176[CrossRef]
HodgkinJ.,
KaiserD.
1979b; Genetics of gliding motility in Myxococcus xanthus (Myxobacterales): two gene systems control movement. Mol Gen Genet 171:177–191[CrossRef]
HumphreyB. A.,
DicksonM. R.,
MarshallK. C.
1979; Physicochemical and in situ observations on the adhesion of gliding bacteria to surfaces. Arch Microbiol 120:231–238[CrossRef]
LünsdorfH.ReichenbachH.
1989; Ultrastructural details of the apparatus of gliding motility of Myxococcus fulvus (Myxobacterales. J Gen Microbiol 135:1633–1641
MathieuO.,
ClaassenH.,
WeibelE. R.
1978; Differential effect of glutaraldehyde and buffer osmolarity on cell dimensions: a study on lung tissue. J Ultrastruct Res 63:20–24[CrossRef]
PateJ. L.,
ChangL.-Y. E.
1979; Evidence that gliding motility in procaryotic cells is driven by rotary assemblies in the cell envelopes. Curr Microbiol 2:59–64[CrossRef]
QuallsG. T.,
StephensK.,
WhiteD.
1978; Morphogenetic movements and multicellular development in the fruiting myxobacterium Stigmatella aurantiaca . Dev Biol 66:270–274[CrossRef]
ReichenbachH.
1980; Saprospira grandis (Leucotrichales) –Wachstum und Bewegung. Film E 2424 des IWF, Göttingen. Sekt Biol, Ser. 13, Nr. 26/E 2424:1–21
RidgwayH.
1977; Source of energy for gliding motility in Flexibacter polymorphus : effects of metabolic and respiratory inhibitors on gliding movement. J Bacteriol 131:544–556
StackebrandtE.
FEMS Symposium 291985; Phylogeny and phylogenetic classification of procaryotes. In Evolution of Procaryotes pp 309–334 Edited by
SchleiferK. H.,
StackebrandtE.
London: Academic Press;