1887

Abstract

The heat-stable toxin (ST) produced by enterotoxigenic strains causes diarrhoea by altering the fluid secretion in intestinal epithelial cells. Here, the effectiveness of a flagellin fusion protein of containing a 19-amino-acid sequence derived from the ST sequence (FLA–ST) in generating antibodies capable of neutralizing the toxic activity of ST was evaluated. This fusion protein, and an alternative construction where two cysteine residues in the ST sequence were substituted by alanines (ST), were delivered to the immune system by three distinct strategies: (i) orally, using an attenuated strain expressing FLA–ST; (ii) intraperitoneally, by injection of purified FLA–ST; (iii) orally, using attenuated carrying a eukaryotic expression plasmid (pCDNA3) with the gene encoding FLA–ST. The results showed that the flagellin system can be used as a carrier to generateST-neutralizing antibodies. However, it should be mentioned that humoral immune response against ST was only obtained when the mutated ST sequence was employed. FLA–ST was found to be non-immunogenic when delivered via the oral route with attenuated strains. However, a flagellin antibody response was obtained by immunizing mice with carrying pCDNA3/FLA-ST. Oral immunization with carrying the eukaryotic expression plasmid (pCDNA3/FLA–ST) seems to be a promising method to elicit an appropriate response against fusions to flagellin.

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2001-04-01
2020-04-02
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