1887

Abstract

The genes encoding type IV O antigen glucosylation were characterized from both and . The putative O antigen modification genes from , , were PCR-amplified and introduced into serotype Y strain SFL124. Immunogold labelling and phage sensitivity indicated the presence of both serotype Y and serotype 4a O antigens on the cell surface of the resulting recombinant SFL124 strains, suggesting that only partial serotype conversion was conferred by the genes. The type IV O antigen modification genes were then isolated and characterized from serotype 4a strain NCTC 8296. A 38 kb chromosomal fragment conferred complete conversion to serotype 4a when introduced into SFL124. Sequence analysis of the fragment revealed the presence of three genes, . DNAs homologous to bacteriophage and were located upstream of , suggesting that this region of the NCTC 8296 genome may have originated from a bacteriophage; however, a serotype-converting phage could not be induced from this strain nor from other strains used in this study. Comparison of the GtrIV and GtrIV (o443) proteins revealed that they are 41% identical and 63% similar, which is the highest degree of similarity reported among the O antigen glucosyltransferases.

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2001-04-01
2020-09-29
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