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Microbiology Society logo Microbiology

Volume 147, Issue 4

type A haemagglutinin-positive progenitor toxin (HA-PTX) binds to oligosaccharides containing Galβ1-4GlcNAc through one subcomponent of haemagglutinin (HA1) Free

Abstract

Haemagglutinin (HA) activity of type A 19S and 16S toxins (HA-positive progenitor toxin; HA-PTX) was characterized. HA titres against human erythrocytes of HA-PTX were inhibited by the addition of lactose, D-galactose, -acetyl-D-galactosamine and D-fucose to the reaction mixtures. A direct glycolipid binding test demonstrated that type A HA-PTX strongly bound to paragloboside and some neutral glycolipids, but did not bind to gangliosides. Type A HA-PTX also bound to asialoglycoproteins (asialofetuin, neuraminidase-treated transferrin), but not to sialoglycoproteins (fetuin, transferrin). Although glycopeptidase F treatment of asialofetuin abolished the binding of HA-PTX, endo-α--acetylgalactosaminidase treatment did not. Thus these results can be interpreted as indicating that type A HA-PTX detects and binds to Galβ1-4GlcNAc in paragloboside and the -linked oligosaccharides of glycoproteins. Regardless of neuraminidase treatment, type A HA-PTX bound to glycophorin A which is a major sialoglycoprotein on the surface of erythrocytes. Both native glycophorin A and neuraminidase-treated glycophorin A inhibited the binding of erythrocytes to type A HA-PTX. Since the -linked oligosaccharide of glycophorin A is di-branched and more than 50% of this sugar chain is monosialylated, type A HA-PTX probably bound to the unsialylated branch of the -linked oligosaccharide of glycophorin A and agglutinated erythrocytes. One subcomponent of HA, designated HA1, did not agglutinate native erythrocytes, although it did bind to erythrocytes, paragloboside and asialoglycoproteins in a manner quite similar to that of HA-PTX. These results indicate that type A HA-PTX binds to oligosaccharides through HA1.

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Keyword(s): binding , CBB, Coomassie brilliant blue , glycolipid , glycoprotein , HA+-PTX, haemagglutinin-positive progenitor toxin , HA, haemagglutinin , PAS, periodic acid–Schiff and sugar chain
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2001-04-01
2025-01-14
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/content/journal/micro/10.1099/00221287-147-4-811
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Clostridium botulinum type A haemagglutinin-positive progenitor toxin (HA+-PTX) binds to oligosaccharides containing Galβ1-4GlcNAc through one subcomponent of haemagglutinin (HA1)
Microbiology 147, 811 (2001); https://doi.org/10.1099/00221287-147-4-811
/content/journal/micro/10.1099/00221287-147-4-811
/content/journal/micro/10.1099/00221287-147-4-811
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