1887

Abstract

The precise start points for transcription of the and of the / genes of the β-lactamase operon have been determined by primer extension analysis. Consequently the overlapping promoter sequences were deduced. Northern blots showed that the synthesis of the 2100 nt mRNA from is inducible and that a probe hybridized to the same mRNA as the probe. The gene , encoding chloramphenicol acetyltransferase, was fused separately to the and / promoters, and used to compare their strengths. The promoter for is about six times stronger than that for / and the synthesis of chloramphenicol acetyltransferase from both promoters is inducible, supporting the results from the Northern blots.

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2001-04-01
2020-03-29
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