1887

Abstract

ATCC14028 readily multiplies in professional phagocytes and is highly virulent in mice. Mutants lacking thymidylate synthase activity () were isolated and shown to be strictly dependent on thymidine monophosphate precursors in the growth medium. The mutants were found to be virtually incapable of intracellular growth and survival , both in macrophage-like cell line P338D and in the human epithelial cell line Hep-2, and their virulence was impaired in BALB/c mice. Intraperitoneal immunization of mice with two doses of live provided protection against a challenge with 10 times the 50% lethal dose of the virulent parent strain.

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2001-03-01
2019-12-07
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