1887

Abstract

When grown in the presence of sunflower cell walls, , an ubiquitous necrotrophic fungus, secretes several acid proteases including a non-aspartyl protease. The gene , encoding an acid protease, has been cloned and sequenced. The intronless ORF encodes a preproprotein of 252 aa and a mature protein of 200 residues. expression of is subject to several transcriptional regulatory mechanisms. Expression induced by plant cell-wall proteins is controlled by both carbon and nitrogen catabolite repression. Glucose on its own represses expression while ammonium repression requires the simultaneous presence of a carbon source. Ambient pH higher than pH 5 overrides induction resulting in full repression of . These transcriptional regulatory mechanisms and the presence of several motifs in the promoter of that may encode binding sites for the regulators CREA, AREA and PacC suggest the involvement of these regulators in the control of expression is expressed during sunflower cotyledon infection. Expression is low at the beginning of infection but increases suddenly at the stage of necrosis spreading. Comparison of and expression suggests that glucose and nitrogen starvation together with acidification can be considered as key factors controlling gene expression during pathogenesis.

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2001-03-01
2019-12-11
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