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Volume 147, Issue 3

Transcription of and during growth of under aerobic and microaerobic conditions Free

Abstract

Physiology of the exponential and stationary phase of growth, under both aerobic and microaerobic conditions, of and its isogenic mutants ::Km, ::Tn, Δ and Δ was studied using transcriptional fusions with the and genes. In the wild-type strain, transcription was greater under aerobic than under microaerobic conditions, whereas transcription of was suppressed by aerobiosis. Under aerobic conditions, no interaction between NuoG, CydA, ArcA and RpoS was detected. Under microaerobic conditions, was suppressed in the mutant as compared with the wild-type strain, but it was overexpressed in the and mutants. A deletion in the gene, on the other hand, resulted in non-restricted, increased expression in stationary-phase cultures under microaerobic conditions. Based on the transcription in the mutant the authors propose that the decrease in the NADH:NAD ratio that occurs when carbon sources become limiting serves as a signal for increased transcription, while active respiration catalysed by CydA and controlled by ArcA downregulates transcription. When, finally, the RpoS-controlled stationary phase of growth is reached, is suppressed in an RpoS-dependent fashion. Transition into stationary phase under microaerobic conditions is thus controlled by coordinated action of the RpoS and ArcA regulators, depending on subtle changes in the environment.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): luxAB transcriptional fusion , RLU, relative luminescence units (luminscence per OD600 unit) , rpoS/arcA , starvation and stationary phase of growth
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2001-03-01
2024-04-19
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Transcription of arcA and rpoS during growth of Salmonella typhimurium under aerobic and microaerobic conditions
Microbiology 147, 701 (2001); https://doi.org/10.1099/00221287-147-3-701
/content/journal/micro/10.1099/00221287-147-3-701
/content/journal/micro/10.1099/00221287-147-3-701
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