1887

Abstract

The gene of ATCC13032 comprises 438 nucleotides and encodes a protein of 145 amino acids with a molecular mass of 153 kDa. The amino acid sequence revealed extensive similarities to the large ribosomal subunit protein L11 from several Gram-positive and Gram-negative bacteria. The gene is located downstream of , representing part of the protein export apparatus, and of , encoding a transcription antiterminator protein. The gene is followed by an ORF homologous to encoding the 50S ribosomal protein L1. Northern analysis revealed that transcription of the cluster resulted in two different transcripts of 15 and 06 kb. The 15 kb transcript corresponds to the entire cluster and the short transcript originates from the gene. A mutant strain carrying a 12 bp in-frame deletion within , which resulted in the loss of the tetrapeptide Pro-Ala-Leu-Gly in the L11 protein, was constructed. The mutant failed to accumulate (p)ppGpp in response to amino acid starvation and exhibited an increased tolerance to the antibiotic thiostrepton. Evidently, the gene is required for (p)ppGpp accumulation upon nutritional starvation.

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2001-03-01
2019-10-23
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