1887

Abstract

The operon of encodes cytochrome , a terminal oxidase in the aerobic respiratory chain. The high oxygen affinity of this oxidase explains its increased synthesis under low-oxygen conditions. Expression of the operon is controlled by the ArcA/ArcB two-component system and the oxygen-sensing transcriptional regulator Fnr. However, expression is still induced upon entry into stationary phase or following a shift to anaerobic conditions in a mutant deleted for and [Cotter, P. A. & Gunsalus, R. P. (1992), 91, 31–36]. Indeed, such a mutant contains 60% of the wild-type levels of spectrally detectable cytochrome . A possible mechanism to account for this regulation is that changes in negative supercoiling, which occur during a shift to low-oxygen or anaerobic conditions, may contribute to the regulation of the operon. This paper reports several lines of evidence in support of this idea. Firstly, the expression of , and the final level of spectrally detectable cytochrome , is sensitive to inhibitors of DNA gyrase, the enzyme responsible for introducing negative supercoils into DNA. Both nalidixic acid and novobiocin reduce expression in a concentration-dependent manner. Secondly, in a mutant, defective in DNA gyrase activity, expression of is reduced to a basal level that is no longer sensitive to the oxygen status. Both gyrase inhibitors and the mutation reduce expression in a strain deleted for and , indicating that their effects are not mediated indirectly through ArcA or Fnr, but rather that they are likely to be direct effects on expression. In conclusion, the authors have shown that changes in DNA supercoiling play a role in the induction of expression and may provide a general way of increasing cytochrome levels in the cell in response to environmental stress.

Keyword(s): arcA , DNA gyrase , fnr , oxygen and respiration
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2001-03-01
2020-03-28
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