1887

Abstract

(now ) is a Gram-negative soil bacterium which can produce yellow pigments. It synthesizes five enzymes that degrade glycosoaminoglycan molecules. The study of this unique bacterium has been limited by the absence of a genetic manipulation system. In this paper, the construction of a conjugation/integration plasmid system and a broad-host-range plasmid, both of which contain a functional selective marker created by placing the trimethoprim resistance gene, , under the control of the regulatory region is described. Both plasmids were introduced into by conjugation and/or electroporation, and trimethoprim resistant colonies were obtained. Fifty electroporants were obtained per microgram covalently closed circular plasmid DNA. The existence of integrated plasmid DNA was confirmed by Southern hybridization and PCR. The existence of a derivative of the broad-host-range plasmid pBBR1 in was demonstrated by plasmid digestion and Southern hybridization, and by transformation of .

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2001-03-01
2020-09-30
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