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Volume 147, Issue 3

NAD(P)H:menadione oxidoreductase of the amitochondriate eukaryote : a simpler homologue of the vertebrate enzyme

The GenBank accession number for the glQR1-encoding sequence reported in this paper is AF321405.

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Abstract

The amitochondriate eukaryote contains an NAD(P)H:menadione oxidoreductase (EC 1.6.99.2) (glQR) that catalyses the two-electron transfer oxidation of NAD(P)H with a quinone as acceptor. The gene encoding this protein in was expressed in . The purified recombinant protein had an NAD(P)H oxidoreductase activity, with NADPH being a more efficient electron donor than NADH. Menadione, naphthoquinone and several artificial electron acceptors served as substrate for the enzyme. glQR shows high amino acid similarity to its homologues in vertebrates and also to a series of hypothetical proteins from bacteria. Although glQR is considerably smaller than the mammalian enzymes, three-dimensional modelling shows similar arrangement of the secondary structural elements. Most amino acid residues of the mammalian enzymes that participate in substrate binding or catalysis are conserved. Conservation of these features and the similarity in substrate specificity and in susceptibility to inhibitors establish glQR as an authentic member of this protein family.

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Keyword(s): , apparent Michaelis constant , DCIP, 2,6-dichorophenolindophenol , DT-diaphorase , Giardia lamblia , glQR1, G. lamblia QR1 , hQR1, QR1 from human , hQR2, QR type 2 from human , menadione oxidoreductase , MT, methyl red , MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide , QR, NAD(P)H:menadione oxidoreductase , QR1, QR type 1 , rQR1, QR1 from rat and SOD, superoxide dismutase
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2001-03-01
2024-04-19
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NAD(P)H:menadione oxidoreductase of the amitochondriate eukaryote Giardia lamblia: a simpler homologue of the vertebrate enzymeThe GenBank accession number for the glQR1-encoding sequence reported in this paper is AF321405.
Microbiology 147, 561 (2001); https://doi.org/10.1099/00221287-147-3-561
/content/journal/micro/10.1099/00221287-147-3-561
/content/journal/micro/10.1099/00221287-147-3-561
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