RT Journal Article SR Electronic(1) A1 Nishigawa, Hisashi A1 Miyata, Shin-ichi A1 Oshima, Kenro A1 Sawayanagi, Toshimi A1 Komoto, Akihiro A1 Kuboyama, Tsutomu A1 Matsuda, Izumi A1 Tsuchizaki, Tsuneo A1 Namba, ShigetouYR 2001 T1 In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteinsThe GenBank accession number for EcOYW1 is AB010426. JF Microbiology, VO 147 IS 2 SP 507 OP 513 DO https://doi.org/10.1099/00221287-147-2-507 PB Microbiology Society, SN 1465-2080, AB A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7·0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-2-507