%0 Journal Article %A Nishigawa, Hisashi %A Miyata, Shin-ichi %A Oshima, Kenro %A Sawayanagi, Toshimi %A Komoto, Akihiro %A Kuboyama, Tsutomu %A Matsuda, Izumi %A Tsuchizaki, Tsuneo %A Namba, Shigetou %T In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteinsThe GenBank accession number for EcOYW1 is AB010426. %D 2001 %J Microbiology, %V 147 %N 2 %P 507-513 %@ 1465-2080 %R https://doi.org/10.1099/00221287-147-2-507 %K extrachromosomal DNA %K TGMV, tomato golden mosaic virus %K Vaccinium witches’ broom %K SCWL, sugar cane white leaf %K AY, aster yellows %K OY-W, wild-type onion yellows phytoplasma %K geminivirus %K phytoplasma %K OY-M, mild symptom line of onion yellows %K Rep protein %K OY, onion yellows %I Microbiology Society, %X A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7·0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-2-507