@article{mbs:/content/journal/micro/10.1099/00221287-147-2-507, author = "Nishigawa, Hisashi and Miyata, Shin-ichi and Oshima, Kenro and Sawayanagi, Toshimi and Komoto, Akihiro and Kuboyama, Tsutomu and Matsuda, Izumi and Tsuchizaki, Tsuneo and Namba, Shigetou", title = "In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteinsThe GenBank accession number for EcOYW1 is AB010426.", journal= "Microbiology", year = "2001", volume = "147", number = "2", pages = "507-513", doi = "https://doi.org/10.1099/00221287-147-2-507", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-2-507", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "extrachromosomal DNA", keywords = "TGMV, tomato golden mosaic virus", keywords = "Vaccinium witches’ broom", keywords = "SCWL, sugar cane white leaf", keywords = "AY, aster yellows", keywords = "OY-W, wild-type onion yellows phytoplasma", keywords = "geminivirus", keywords = "phytoplasma", keywords = "OY-M, mild symptom line of onion yellows", keywords = "Rep protein", keywords = "OY, onion yellows", abstract = "A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7·0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes.", }