1887

Abstract

A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 70 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes.

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2001-02-01
2020-01-24
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References

  1. Altschul S. F, Gish W, Miller W, Myers E. W, Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol215:403–410[CrossRef]
    [Google Scholar]
  2. Bertaccini A, Davis R. E, Lee I.-M, Conti M, Dally E. L, Douglas S. M. 1990; Detection of chrysanthemum yellows mycoplasmalike organism by dot hybridization and Southern blot analysis. Plant Dis74:40–43[CrossRef]
    [Google Scholar]
  3. Davis M. J, Tsai J. H, Cox R. L, McDaniel L. L, Harrison N. A. 1988; Cloning of chromosomal and extrachromosomal DNA of the mycoplasmalike organism that causes maize bushy stunt disease. Mol Plant–Microbe Interact4:295–302
    [Google Scholar]
  4. Garnier T, Cole S. T. 1988; Identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pIP404 from Clostridium perfringens.. Plasmid19:151–160[CrossRef]
    [Google Scholar]
  5. Goodwin P. H, Xue B. G, Kuske C. R, Sears M. K. 1994; Amplification of plasmid DNA to detect plant pathogenic mycoplasmalike organism. Ann Appl Biol124:27–36[CrossRef]
    [Google Scholar]
  6. Gundersen D. E, Lee I.-M, Rehner S. A, Davis R. E, Kingsbury D. T. 1994; Phylogeny of mycoplasmalike organisms (phytoplasmas), a basis for their classification. J Bacteriol176:5244–5254
    [Google Scholar]
  7. Harrison N. A, Tsai J. H, Bourne C. M, Richardson P. A. 1991; Molecular cloning and detection of chromosomal and extrachromosomal DNA of mycoplasmalike organisms associated with witches’ broom disease of pigeon pea in Florida. Mol Plant–Microbe Interact4:300–307[CrossRef]
    [Google Scholar]
  8. Kenton A, Khashoggi A, Parokonny A, Bennett M. D, Lichtenstein C. 1995; Chromosomal location of endogenous geminivirus-related DNA sequences in Nicotiana tabacum L. . Chromosome Res3:346–350[CrossRef]
    [Google Scholar]
  9. Kirkpatrick B. C. 1992; Mycoplasma-like organisms: plant and invertebrate pathogens. In The Prokaryotes pp.4050–4067Edited by Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H.. New York: Springer;
    [Google Scholar]
  10. Koonin E. V, Ilyina T. V. 1992; Geminivirus replication proteins are related to prokaryotic plasmid rolling circle DNA replication initiator proteins. J Gen Virol73:2763–2766[CrossRef]
    [Google Scholar]
  11. Kuboyama T, Huang C.-C, Lu X, Sawayanagi T, Kanazawa T, Kagami T, Matsuda I, Tsuchizaki T, Namba S. 1998; A plasmid isolated from phytopathogenic onion yellows phytoplasma and its heterogeneity in the pathogenic phytoplasma mutant. Mol Plant–Microbe Interact11:1031–1037[CrossRef]
    [Google Scholar]
  12. Kuske C. R, Kirkpatrick B. C. 1990; Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism. J Bacteriol172:1628–1633
    [Google Scholar]
  13. Lee I.-M, Davis R. E. 1983; Phloem-limited prokaryotes in sieve elements isolated by enzyme treatment of diseased plant tissues. Phytopathology73:1540–1543[CrossRef]
    [Google Scholar]
  14. McCoy R. E, Caudwell A, Chang C. J. 15 other authors 1989; Plant disease associated with mycoplasma-like organisms. In The Mycoplasmas vol 5 pp.546–640Edited by Whitcomb R. F., Tully J. G.. New York: Academic Press;
    [Google Scholar]
  15. Maniloff J. 1988; Mycoplasma viruses. Crit Rev Microbiol15:339–389[CrossRef]
    [Google Scholar]
  16. Nakashima K, Hayashi T. 1997; Sequence analysis of extrachromosomal DNA of sugarcane white leaf phytoplasma. Ann Phytopathol Soc Jpn63:21–25[CrossRef]
    [Google Scholar]
  17. Nur I, Leblanc D. J, Tully J. G. 1987; Short, interspersed, and repetitive DNA sequences in Spiroplasma species. Plasmid17:110–116[CrossRef]
    [Google Scholar]
  18. Orozco B. M, Hanley-Bowdoin L. 1998; Conserved sequence and structural motifs contribute to the DNA binding and cleavage activities of a geminivirus replication protein. J Biol Chem273:24448–24456[CrossRef]
    [Google Scholar]
  19. Panopoulos N, Peet R. C. 1985; The molecular genetics of plant pathogenic bacteria and their plasmids. Annu Rev Phytopathol23:381–419[CrossRef]
    [Google Scholar]
  20. Razin S. 1985; Molecular biology and genetics of mycoplasmas (Mollicutes. Microbiol Rev49:419–455
    [Google Scholar]
  21. Rekab D, Carraro L, Schneider B, Chen J, Chang C. J, Locci R., Firrao G., Seemüller E.. 1999; Geminivirus-related extrachromosomal DNAs of the X-clade phytoplasmas share high sequence similarity. Microbiology145:1453–1459[CrossRef]
    [Google Scholar]
  22. Renaudin J, Bove J. M. 1994; SpV1 and SpV4, spiroplasma viruses with circular, single-stranded DNA genomes, and their contribution to the molecular biology of spiroplasmas. Adv Virus Res44:429–463
    [Google Scholar]
  23. Sambrook J, Fritsch E. F, Maniatis T. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Sears B. B, Lim P.-O, Holland N, Kirkpatrick B. C, Klomparens K. L. 1989; Isolation and characterization of DNA from a mycoplasmalike organism. Mol Plant-Microbe Interact2:175–180[CrossRef]
    [Google Scholar]
  25. Shiomi T, Tanaka M, Wakiya H, Zenbayashi R. 1996; Occurrence of welsh onion yellows. Ann Phytopathol Soc Jpn62:258–260[CrossRef]
    [Google Scholar]
  26. Shiomi T, Tanaka M, Sawayanagi T, Yamamoto S, Tsuchizaki T, Namba S. 1998; A symptomatic mutant of onion yellows phytoplasma derived from a greenhouse-maintained isolate. Ann Phytopathol Soc Jpn64:501–505[CrossRef]
    [Google Scholar]
  27. Thompson J. D, Higgins D. G, Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res22:4673–4680[CrossRef]
    [Google Scholar]
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