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Volume 147, Issue 2

mutants defective in siderophore production, motility, serum resistance and catalase activity

The GenBank accession numbers for the sequences determined in this work are AF324338–AF324342 (Table 3 T3 ).

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Abstract

is a Gram-negative bacterium that causes a systemic infection, edwardsiellosis, in fish. The virulence factors of this pathogen and its genetic determinants have not been systematically examined. In this study, Tn transposon mutagenesis was used to construct a library of 440 alkaline phosphatase (PhoA) fusion mutants from a total of 400000 transconjugants derived from PPD130/91. This library included genes for secreted and membrane-associated proteins normally involved in virulence. The library was screened for four virulence factors: siderophore production, motility, serum resistance and catalase production. Eight mutants deficient in one or more of these phenotypes were grouped into four classes. They were further characterized for their stimulation of reactive oxygen intermediate production by fish phagocytes, for their adhesion to and internalization into EPC (epithelioma papillosum of carp) cells, and for attenuation of virulence in blue gourami. Mutants 2A and 34 were highly attenuated in fish, with LD values about 10 times higher than for the wild-type. These strains had mutations in the genes encoding arylsulfate sulfotransferase (mutant 2A) and a catalase precursor protein (mutant 34). One hyperinvasive/adhesive mutant and four mutants that were pleiotropic and slightly attenuated in fish were also isolated.

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  • Accepted:
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Keyword(s): CAS, chrome azurol S , EPC, epithelioma papillosum of carp , fish pathogen , ROI, reactive oxygen intermediates , transposon mutagenesis and virulence genes
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2001-02-01
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Edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activityThe GenBank accession numbers for the sequences determined in this work are AF324338–AF324342 (Table 3T3).
Microbiology 147, 449 (2001); https://doi.org/10.1099/00221287-147-2-449
/content/journal/micro/10.1099/00221287-147-2-449
/content/journal/micro/10.1099/00221287-147-2-449
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