1887

Abstract

The regulation of the divergent promoters of the genes in ATCC 17933, in which encodes a quinoprotein ethanol dehydrogenase and codes for a cytochrome , was studied. Using transcriptional fusions, promoter activity during growth on several substrates was measured. These promoter-probe vectors were also used to identify regulatory mutants defective in induction. Transcription from both and was reduced significantly in four mutants. Two other mutants showed transcription from that was reduced, but higher than wild-type transcription from . The genes that are needed for promoter induction were sequenced and found to encode a two-component regulatory system: a histidine sensor kinase, which lacks a transmembrane helical N-terminus and is presumably located in the cytoplasm, and a response regulator. The phenotypic characterization and restoration of the wild-type behaviour of the different regulatory mutants produced by different cosmids and subclones indicate that six different genes may be involved in regulating ethanol oxidation in

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2001-02-01
2020-04-07
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